CD11c Antibody | 8A2

CD11c Antibody | 8A2 gallery image 1

Staining of rat peripheral blood lymphocytes with Mouse anti Rat CD11c:FITC (MCA1441F)

CD11c Antibody | 8A2 gallery image 2

Figure A. FITC conjugated mouse anti rat CD11c (MCA1441F) and RPE conjugated mouse IgG1 isotype control (MCA1209PE). Figure B. FITC conjugated mouse anti rat CD11c (MCA1441F) and RPE conjugated mouse anti rat CD172a (MCA620PE). All experiments performed on rat peritoneal macrophages.

CD11c Antibody | 8A2 gallery image 3

Figure A. PE conjugated mouse anti rat CD172a (MCA620PE) and FITC conjugated mouse IgG2a isotype control (MCA1210FITC). Figure B. PE conjugated mouse anti rat CD172a (MCA620PE) and FITC conjugated mouse anti rat CD11c (MCA1441F). All experiments performed on rat peritoneal macrophages.

CD11c Antibody | 8A2 gallery image 4

Published customer image:
Mouse anti RatCD11c antibody, clone 8A2 (MCA1441) used for the evaluation of CD11c expression in rat eye sections by immunofluorescence.
Image caption:
Immunohistochemistry staining for CD4+, CD8+, CD11c+, CD163+, CD54+, and CD25+ cells at graft-host junction for different groups.
The grafts treated with the PA microfilms or PA eye drops had less dense and diffuse cell infiltration in comparison with the allogeneic control grafts. The syngeneic grafts were nearly free of immunologic cell infiltration. G: Graft. H: Host. Original magnification ×100. Scale bar: 100 μm.

From: Liu Y-C, Peng Y, Lwin NC, Venkatraman SS, Wong TT, Mehta JS (2013)
A Biodegradable, Sustained-Released, Prednisolone Acetate Microfilm Drug Delivery System Effectively Prolongs Corneal Allograft Survival in the Rat Keratoplasty Model.
PLoS ONE 8(8): e70419.

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  • Mouse anti Rat CD11c
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1441C, F, IF, IPdatasheet pdfdatasheet pdf0.25 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Rat CD11c antibody, clone 8A2 recognizes the rat CD11c, also know as Integrin Alpha-X or Integrin Alpha-D, cell surface antigen, expressed by some myeloid cells and dendritic cells.

      Mouse anti Rat CD11c antibody, clone 8A2 immunoprecipitates a 130-140/90 kDa heterodimer and modestly inhibits macrophage adherence on plastic (by around 20-30%).
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Rat alveolar macrophages.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      cell adhesion
      integrin-mediated signaling pathway
      integrin complex
      protein heterodimerization activity
      receptor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunohistology - Frozen10ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD11c Antibody Formats

    Formats Clone Applications Sizes available
    CD11c Antibody : Purified 8A2 C, F, IF, IP 0.25 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative ControlMCA1210100 TestsF

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry


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