Caspr1 Antibody | 6152

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Caspr1 Antibody | 6152 gallery image 1

Caspr1 detected with Human anti Rat Caspr1:HRP (HCA091P)

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Caspr1 Antibody | 6152 gallery image 2

Immunofluorescence detection of mouse Caspr1 (green) at paranodes using Human anti Rat anti Caspr1 clone AbD6152, bilaterally flanking node of Ranvier indicated by Ankyrin G staining (red)
Picture provided courtesy of B. Ranscht, Burnham Institute for Medical Research

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  • Human anti Caspr1:Biotin
  • Human anti Caspr1
  • Human anti Caspr1
  • Human anti Caspr1:HRP
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    6152
  • Isotype
    HuCAL Fab bivalent
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA091BC, E, WBdatasheet pdfdatasheet pdf0.1 mg
    HCA091B
    HCA091C, E, WBdatasheet pdfdatasheet pdf0.1 mg
    HCA091
    HCA091PC, E, WBdatasheet pdfdatasheet pdf0.1 mg
    HCA091P
    HCA091AC, E, WBdatasheet pdfdatasheet pdf50 µg
    HCA091A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Caspr1 antibody, clone 6152 recognizes rat contactin-associated protein 1 (Caspr1), also known as neurexin 4 and paranodin, which is a 190kDa axonal transmembrane molecule. Caspr1 is a primarily expressed in myelinated nerves of the central and peripheral nervous system. It is a constituent of paranodal regions flanking the nodes of Ranvier and is believed to play an important role in paranodal junction formation.
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      HumanExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid, conjugated to Horseradish Peroxidase (HRP).
    • Reconstitution
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
    • Preparation
      metal chelate affinity chromatography
      metal chelate affinity chromatography
      metal chelate affinity chromatography
      Metal chelate affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.01% Thiomersal
      1% Bovine Serum Albumin
    • Immunogen
      Fusion protein corresponding to the C-terminal region of rat Caspr1.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.1mg/ml
      Total protein concentration 0.1mg/ml
      Total protein concentration 0.1mg/ml
      Total protein concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of reconstitution.
      12 months from date of reconstitution.
      12 months from date of despatch.
    • GO Terms
      paranode region of axon
      signal transduction
      SH3 domain binding
      receptor binding
      integral to membrane
      neuron projection development
      myelination
      cell adhesion
      plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/1000
      Immunohistology - Frozen
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen
      Western Blotting1/1001/500
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen
      Western Blotting1/1001/500
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/500
      Immunohistology - Frozen
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
      Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
    • ELISA
      Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Mouse brain
    • Histology Positive Control Tissue
      Mouse brain
    • Histology Positive Control Tissue
      Mouse brain
    • Histology Positive Control Tissue
      Mouse brain
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      HCA091B detects a band of approximately 190kDa in mouse brain cell lysates.
    • Western Blotting
      HCA091 detects a band of approximately 190kDa in mouse brain cell lysates.
    • Western Blotting
      HCA091A detects a band of approximately 190kDa in mouse brain cell lysates.
    • Western Blotting
      HCA091P detects a band of approximately 190kDa in mouse brain cell lysates.
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Caspr1 Antibody Formats

    Formats Clone Applications Sizes available
    Caspr1 Antibody : Biotin 6152 C, E, WB 0.1 mg
    Caspr1 Antibody : Purified 6152 C, E, WB 50 µg | 0.1 mg
    Caspr1 Antibody : HRP 6152 C, E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Mouse anti c-Myc:Alexa Fluor® 488MCA2200A488100 Tests/1mlF *
      MCA2200A488
      Mouse anti Histidine Tag:Alexa Fluor® 647MCA1396A6471 mlF
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      Recommended Negative Isotype Control

        Useful Reagents

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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          BUF057A
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          BUF062A
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          Recommended Positive Controls

            Histology Controls

              Mouse brain
              Mouse brain
              Mouse brain
              Mouse brain

              References

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