Calcitonin Receptor Antibody

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Calcitonin Receptor Antibody gallery image 1

Published customer image:
Rabbit anti Rat Calcitonin Receptor antibody (AHP635) used for the detection of calcitonin receptor (CTR) on murine myofibres by immunofluorescence.
Image caption:
Caveolin-1, integrin α7 and CTR are expressed by Pax7+ve quiescent satellite cells in their niche on the myofibre. Satellite cells in their niche on the surface of freshly isolated EDL myofibres were co-immunostained for Pax7 (green) and either caveolin-1 (a - red), integrin α7 (b - red) or CTR (c - red). All three proteins were expressed in the plasma membrane of Pax7+ve satellite cells (arrowed), clearly revealing their bi-polar morphology. Myofibres were also examined from the masseter of the jaw, and again Pax7+ve satellite cells expressed all three markers (caveolin-1 shown as an example in d). All nuclei were counterstained with DAPI (blue), revealing the presence of the myonuclei of the myofibres on which the satellite cells were located. Scale bar represents 20 μm.

From: Gnocchi VF, White RB, Ono Y, Ellis JA, Zammit PS (2009) Further Characterisation of the Molecular Signature of Quiescent and Activated Mouse Muscle Satellite Cells.
PLoS ONE 4(4): e5205.

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Calcitonin Receptor Antibody gallery image 2

Published customer image:
Rabbit anti Rat Calcitonin Receptor antibody (AHP635) used for the detection of calcitonin receptor (CTR) on murine myofibres by immunofluorescence.
Image caption:
Jagged-1 is induced when satellite cells are activated. EDL myofibres were cultured in suspension for 48 hrs in plating medium to elicit the activation and proliferation of satellite cells (arrows). Activated and proliferating satellite cells were identified by immunostaining for MyoD (green), and Caveolin-1 (a - red), integrin α7 (b - red) and CTR (c - red) remained expressed in the plasma membrane of activated satellite cells. The continued presence of both emerin (red, d) and lamin A/C (green, e) in the nuclear envelope of activated satellite cells expressing MyoD (green in d, red in e) was also noted. Moreover, we found that the Notch ligand Jagged-1 (red) was induced upon activation, in MyoD+ve (green) satellite cells (f). Nuclei were counterstained with DAPI (blue). Scale bar represents 20 μm.

From: Gnocchi VF, White RB, Ono Y, Ellis JA, Zammit PS (2009) Further Characterisation of the Molecular Signature of Quiescent and Activated Mouse Muscle Satellite Cells.
PLoS ONE 4(4): e5205.

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  • Rabbit anti Rat Calcitonin Receptor
  • Rabbit anti Rat Calcitonin Receptor
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP635C, E, IF, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP635
    AHP635TC, E, IF, P *datasheet pdfdatasheet pdf10 µg
    AHP635T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Rat Calcitonin Receptor antibody recognizes the calcitonin receptor in human, mouse and rat. The calcitonin receptor is a seven transmembrane domain cell surface protein which is coupled to G protein messenger systems. Rabbit anti Rat Calcitonin Receptor antibody recognizes an epitope within the cytoplasmic domain that is common to both C1a and C1b rat isoforms.

      Calcitonin receptor is expressed in kidney, central and peripheral nervous systems and in osteoclasts. It is also expressed in a number of tissues during the development of the embryo and may be important in foetal morphogenesis.
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Humanyes
      Fishyes
      Atlantic Stingrayyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to calcitonin receptor were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      Synthetic peptide derived from a C-terminal sequence of rat calcitonin receptor.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 10 µgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 10 µgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,000
      Immunofluorescence
      Immunohistology - Frozen1/101/50
      Western BlottingReducing conditions
      Immunohistology - Paraffin(1)1/101/50
      (1)
      The use of 4% paraformaldehyde fixation, rather than formalin fixation is recommended.

      A sensitive detection system is also recommended. e.g. A catalysed signal amplification system.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,000
      Immunofluorescence
      Immunohistology - Frozen1/101/50
      Western BlottingReducing conditions
      Immunohistology - Paraffin(1)1/101/50
      (1)
      The use of 4% paraformaldehyde fixation, rather than formalin fixation is recommended.

      A sensitive detection system is also recommended. e.g. A catalysed signal amplification system.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Kidney, cultured osteoclasts.
    • Histology Positive Control Tissue
      Kidney, cultured osteoclasts
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Calcitonin Receptor Antibody Formats

    Formats Applications Sizes available
    Calcitonin Receptor Antibody : Purified C, E, IF, P *, WB 10 µg | 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Kidney, cultured osteoclasts.
              Kidney, cultured osteoclasts
              • Application NameReference Images
                Immunohistology - Paraffin
                Western Blotting

              References

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