Calcitonin Receptor Antibody | 21/00-A3-14-13

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  • Mouse anti Rat Calcitonin Receptor (C1B Isoform)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    21/00-A3-14-13
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2192E, Pdatasheet pdfdatasheet pdf0.1 mg
    MCA2192
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Rat calcitonin receptor antibody, clone 21/00-A3-14-13 recognizes the rat calcitonin receptor, a 482 amino acid ~55 kDa multi pass transmembrane membrane glycoprotein with seven transmembrane domains that is coupled to G protein messenger systems. Clone 21/00 recognises an epitope within extracellular domain that is unique to the C1b isoform.

      Calcitonin receptor is expressed in kidney, central and peripheral nervous systems and on osteoclasts. It is also expressed in a number of tissues during the development of the embryo and may be important in foetal morphogenesis.

      Two isoforms of the rat calcitoni receptor produced by alternative splicing have been described. Mouse anti Rat calcitonin receptor antibody, clone 21/00-A3-14-13 binds to the canonical isoform 1, binding to the truncated isoform 2 has not been evaluated (UniProt : P32214).
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Synthetic peptide derived from sequence of rat calcitonin receptor.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1,000
      Immunohistology - Paraffin1/501/500

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin embedded sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
    • Histology Positive Control Tissue
      Kidney
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Calcitonin Receptor Antibody Formats

    Formats Clone Applications Sizes available
    Calcitonin Receptor Antibody : Purified 21/00-A3-14-13 E, P 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Kidney

              References

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