Aquaporin 2 (pSer261) Antibody

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Aquaporin 2 (pSer261) Antibody gallery image 1

Western blot of rat kidney lysate showing phosphospecific immunolabeling of the ~29 kDa and ~37 kDa glycosylated form of the AQP2 protein phosphorylated at Ser261

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  • Aquaporin 2 (pSer261) Antibody thumbnail image 1
  • Rabbit anti Aquaporin 2 (pSer261)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1299WB*datasheet pdfdatasheet pdf0.1 ml
    AHP1299
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Rat aquaporin 2 (pSer261) antibody recognizes aquaporin 2 (AQP-2), also known as ADH water channel, aquaporin-CD (AQP-CD) and collecting duct water channel protein (WCH-CD), when phosphorylated at serine 261.

      AQP2 provides the plasma membranes of the renal collecting duct with a high permeability channel to enable the movement of water across an osmotic gradient, under hormonal regulation by vasopressin. In addition to the serine 256 phosphorylation site, a novel phosphorylated serine 261 site on AQP2 exists, which decreases in expression, in response to vasopressin.

      Gene mutations in AQP2 result in hereditary nephrogenic diabetes insipidus in humans, and acquired diabetes insipidus can also occur through a decrease in the expression of the AQP2 gene, following lithium treatment. Expression of the AQP2 gene is increased during pregnancy and other conditions associated with water retention.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting(1)1/1000
      (1)
      For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1299 detects bands of approximately 29kDa and 37kDa (glycosylated form) in rat kidney cell lysates.
    • Instructions For Use

    Additional Aquaporin 2 (pSer261) Antibody Formats

    Formats Applications Sizes available
    Aquaporin 2 (pSer261) Antibody : Purified WB* 0.1 ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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