100% secure
Email this product page
Print this page

Ig Antibody | 2C7

Ig Antibody | 2C7 gallery image 1

Purchased this product? Why not be the first to submit a review and upload an image?

  • Ig Antibody | 2C7 thumbnail image 1
  • Mouse anti Rainbow Trout Ig
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5976C, E, IF, WBdatasheet pdfdatasheet pdf0.5 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Rainbow Trout Ig, clone 2C7 recognizes immunoglobulin heavy chain from Rainbow Trout (Oncorhynchus mykiss), additionally clone 2C7 recognizes Ig from closely related piscine species belonging to the Oncorhynchus genera but not from Atlantic salmon (Salmo salar) (Kibenge, M.T., et. al. 2002) or more distantly related species such as Zebra Fish (Danio rerio).

      Rainbow trout is one of a number of salmonid fish species widely used in the aquaculture industry in many countries. As with other farmed salmonid species, the close proximity of many individuals in a confined area leads to increased incidences of disease caused by a range of fungal, protozoan, bacterial and viral pathogens. The development of vaccine and treatment regimes is essential to combat these infections.

      Clone 2C7 may be used to detect bacterial and viral infections in trout and salmon, and to identify the presence of unknown parasites indirectly by measurement and monitoring of serum immunoglobulins. Monoclonal antibodies specific to salmonid Ig have been demonstrated to be of use in measuring the efficacy of salmonid vaccines (Rømer Villumsen, K., et. al. 2012 and Chettri, J.K., et. al. 2013).
    • Intended Use
    • Target Species
      Rainbow Trout
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Atlantic Salmonno
      Chinnock Salmonyes
      Chum Salmonyes
      Coho Salmonyes
      Sockeye Salmonyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Tissue Culture Supernatant - liquid (concentrated)
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Rainbow Trout heavy and light chain immunoglobulins
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.2 - 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse NSO myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Ig Antibody Formats

    Formats Clone Applications Sizes available
    Ig Antibody : Con S/N 2C7 C, E, IF, WB 0.5 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Salmonid IgMCA21820.5 mlE, F, IF, WB
          Rabbit anti Salmonid IgAHP76150 µlE, IF, WB

          Recommended Positive Controls

            Histology Controls


              Further Reading

              Write your review

              You may also be interested in...