Tryptophan Hydroxylase (pSer58) Antibody

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Tryptophan Hydroxylase (pSer58) Antibody gallery image 1

Western blot of recombinant tryptophan hydroxylase in bacterial lysate incubated in the absence and presence of PKA showing phosphospecific immunolabeling of the ~55 kDa protein phosphorylated at Ser58

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  • Rabbit anti Tryptophan Hydroxylase (pSer58)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP918WB*datasheet pdfdatasheet pdf0.1 ml
    AHP918
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti phosphorylated tryptophan hydroxylase antibody recognizes tryptophan hydroxylase (TPH), when phosphorylated at serine 58.

      TPH catalyses the 5-hydroxylation of tryptophan, in the first stage in indolamine biosynthesis (e.g. serotonin and melatonin). The activity of TPH is enhanced by phosphorylation of Ser58, by cAMP dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAMKII).
    • Intended Use
    • Target Species
      Rabbit
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      HumanExpected from Sequence
      BovineExpected from Sequence
      MonkeyExpected from Sequence
      MouseExpected from Sequence
      DogExpected from Sequence
      XenopusExpected from Sequence
      RatExpected from Sequence
      ZebrafishExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to phosphorylated tryptophan hydroxylase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.01%Bovine Serum Albumin
      50%Glycerol
    • Immunogen
      Synthetic phosphopeptide corresponding to an amino acid sequence within Tryptophan Hydroxylase which includes the phosphorylated residue Ser58.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      10mM HEPES pH7.5
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      serotonin biosynthetic process
      aromatic amino acid family metabolic process
      tryptophan 5-monooxygenase activity
      oxidation-reduction process
      amino acid binding
      iron ion binding
    • UniProt
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting(1)1/1000
      (1)
      For the detection of phosphoproteins, threonine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP918 detects a band of approximately 55kDa in Western Blotting.
    • Instructions For Use

    Additional Tryptophan Hydroxylase (pSer58) Antibody Formats

    Formats Applications Sizes available
    Tryptophan Hydroxylase (pSer58) Antibody : Purified WB* 0.1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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