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Sheep antiRabbit tryptophan hydroxylasae antibody used to ientify immunoreactive neurons by immunohistochemistry on cryostat sections.
Influence of M. vaccae or its derivatives on pulmonary cytokine mRNA expression, c-Fos expression in the nTS and c-Fos expression in serotonergic neurons in the dorsal raphe nucleus (DR). (a) Graphs illustrate mean levels, relative to β-actin, of pulmonary IL-1β (IL-1), TNF-α (TNF), and IL-6 mRNA expression 12 h, and 3, 6, 10, and 17 days following i.t. injection of Mv-NC in M. vaccae–preimmunized mice (•) relative to cytokine mRNA expression following i.t. injection of vehicle in M. vaccae–preimmunized mice (O) (12 h time point only), as well as, for comparison, cytokine mRNA expression at the same time points following i.t. injection of OVA-NC in OVA/alum-preimmunized mice (▼) or vehicle in OVA/alum-preimmunized mice (▽) (12 h time point only). (b) Bar graphs illustrate the mean number (±S.E.M.) of c-Fos-ir nuclei in the AP, SolDL, DRC, and DRI in experiment 1. (c) Photographs illustrate nuclear c-Fos immunostaining (blue–black) 12 h following i.t. injection of M. vaccae in M. vaccae–preimmunized mice in the AP and SolDL of the nTS (top two photographs) and serotonergic neurons in the DRC (middle two photographs) and DRI parts (bottom two photographs) of the DR. Tyrosine hydroxylase immunostaining (brown) was used to aid in identification of neuroanatomical subdivisions of the nTS. Tryptophan hydroxylase immunostaining (brown) was used to identify serotonergic neurons in the DRC and DRI. (⇒) c-Fos-immunonegative serotonergic neurons, (→) c-Fos-immunopositive serotonergic neurons. Small black boxes in (c) indicate regions shown at higher magnification in insets. Scale bar=100 μm (c) top row; (c) middle and bottom rows, 25 μm; (c) insets, 12.5 µm. Abbreviations: IL-1, interleukin-1β; (Mv), preimmunization with s.c. injections of heat-killed M. vaccae; Mv-NC, i.t. challenge with sonicated heat-killed Mv-NC; NC, i.t. challenge with NC. ' P≤0.05, compared with M. vaccae–preimmunized, vehicle-injected controls, Fisher's protected LSD test. For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.
From: Lowry CA, Hollis JH, de Vries A, Pan B, Brunet LR, Hunt JR, Paton JF, van Kampen E, Knight DM, Evans AK, Rook GA, Lightman SL. Identification of an immune-responsive mesolimbocortical serotonergic system: potential role in regulation of emotional behavior.
Neuroscience. 2007 May 11;146(2):756-72.