Tryptophan Hydroxylase Antibody

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Published customer image:
Sheep antiRabbit tryptophan hydroxylase antibody used for immunostaining neuroins in rat brain by immunohistochemistry on cryostat sections.
Image caption:
Fos expression in TrpOH-immunostained neurons in the DRN/MnR and ChAT-immunostained neurons in the PPT/LDT. A. Illustrations of brain sections of the DRN and MnR. B. Photomicrograph illustrating an example of Fos expression in TrpOH-immunostained neurons in the DRN and MnR. Scale bars = 100 μm. C and D. Fos expression positive ratio in serotonergic neurons in the DRN, there were no difference among groups. E and F. Fos expression positive ratio in serotonergic neurons in the MnR, there were no difference among groups. G and I. Illustrations of brain sections of the PPT and LDT. H and J. Photomicrograph illustrating an example of Fos expression in ChAT-immunostained neurons in the PPT and LDT. Scale bars = 100 μm. K and L. Fos expression positive ratio in cholinergic neurons in the PPT, there were no difference among groups. M and N. Fos expression positive ratio in cholinergic neurons in the LDT, there were no difference among groups. Data are represented as mean ± SEM.

From: Yu B, Cui SY, Zhang XQ, Cui XY, Li SJ, Sheng ZF, Cao Q, Huang YL, Xu YP, Lin ZG, Yang G, Song JZ, Ding H, Zhang YH. Mechanisms Underlying Footshock and Psychological Stress-Induced Abrupt Awakening From Posttraumatic "Nightmares".
Int J Neuropsychopharmacol. 2015 Nov 20. pii: pyv113.

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Published customer image:
Sheep anti Rabbit tryptophan hydroxylase antibody used to identify neurons in rat brain by immunohistochemistry on cryostat sections
Image caption:
Low magnification photomicrographs illustrating TrpOH/c-Fos-immunostained sections from different rostrocaudal levels of the DR from a control (CO) rat. TrpOH-immunopositive neurons and dendrites can be identified by the brown/orange precipitate within subdivisions of the DR. The subdivisions of the DR analyzed are illustrated by dashed lines (adapted from a standard stereotaxic atlas of the rat brain [42]). Abbreviations: Aq, cerebral aqueduct; DRC, dorsal raphe nucleus, caudal part; DRD, dorsal raphe nucleus, dorsal part; DRI, dorsal raphe nucleus, interfascicular part; DRV, dorsal raphe nucleus, ventral part; DRVL, dorsal raphe nucleus, ventrolateral part; mlf, medial longitudinal fasciculus; xscp, decussation of the superior cerebellar peduncle. Scale bar, 500μm.

From: Bouwknecht JA, Spiga F, Staub DR, Hale MW, Shekhar A, Lowry CA. Differential effects of exposure to low-light or high-light open-field on anxiety-related behaviors: relationship to c-Fos expression in serotonergic and non-serotonergic neurons in the dorsal raphe nucleus.
Brain Res Bull. 2007 Apr 2;72(1):32-43.

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Published customer image:
Sheep anti Rabbit tryptophan hydroxylase antibody used to identify neurons in rat brain by immunohistochemistry on cryostat sections
Image caption:
Photomicrographs illustrating c-Fos-immunopositive nuclei and TrpOH-immunopositive neurons in the mid-rostrocaudal DRVL (-8.18 mm Bregma) of rats exposed to (A) CO, (B) HA, (C) LL, and (D) HL conditions. Black boxes indicate regions shown at higher magnification in insets in the lower right hand corner of each panel. Arrowheads indicate examples of c-Fos-immunopositive cells (blue/black nuclear staining); arrows indicate TrpOH-immunopositive (serotonergic) neurons (brown/orange cytoplasmic staining). c-Fos-immunopositive/TrpOH-immunopositive neurons were rarely observed. bv, blood vessels characteristic of the DRVL region at this rostrocaudal level. Scale bar, 50 μm; inset: 25 μm.

From: Bouwknecht JA, Spiga F, Staub DR, Hale MW, Shekhar A, Lowry CA. Differential effects of exposure to low-light or high-light open-field on anxiety-related behaviors: relationship to c-Fos expression in serotonergic and non-serotonergic neurons in the dorsal raphe nucleus.
Brain Res Bull. 2007 Apr 2;72(1):32-43.

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Tryptophan Hydroxylase Antibody gallery image 4

Published customer image:
Sheep anti Rabbit tryptophan hydroxylase antibody used for the demonstration of immunoreactive neurons in rat brain by immunohistochemistry on cryostat sections.
Image caption:
Low magnification photomicrographs illustrating TrpOH/c-Fos-immunostained sections from different rostrocaudal levels of the midbrain raphe nuclei from a control rat. TrpOH-ir neurons and dendrites can be identified by the brown/orange precipitate within subdivisions of the DR and MnR. The subdivisions of the DR and MnR analyzed are illustrated by dashed lines (adapted from a standard stereotaxic atlas of the rat brain; Paxinos and Watson, 1998). Abbreviations: Aq, cerebral aqueduct; DRC, dorsal raphe nucleus, caudal part; DRD, dorsal raphe nucleus, dorsal part; DRI, dorsal raphe nucleus, interfascicular part; DRV, dorsal raphe nucleus, ventral part; DRVL, dorsal raphe nucleus, ventrolateral part; mlf, medial longitudinal fasciculus; MnR, median raphe nucleus; xscp, decussation of the superior cerebellar peduncle. Scale bar, 500 μm.

From: Hale MW, Hay-Schmidt A, Mikkelsen JD, Poulsen B, Bouwknecht JA, Evans AK, Stamper CE, Shekhar A, Lowry CA. Exposure to an open-field arena increases c-Fos expression in a subpopulation of neurons in the dorsal raphe nucleus, including neurons projecting to the basolateral amygdaloid complex.
Neuroscience. 2008 Dec 10;157(4):733-48.

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Published customer image:
Sheep anti Rabbit tryptophan hydroxylase antibody used for the demonstration of immunoreactive neurons in rat brain by immunohistochemistry on cryostat sections.
Image caption:
Photomicrographs illustrating the effects of exposure to the open-field in low-light, or high-light conditions compared to home cage controls, on c-Fos expression in serotonergic and non-serotonergic neurons in the mid-rostrocaudal dorsal raphe nucleus (-8.18 mm Bregma). Photomicrographs illustrate c-Fos-ir nuclei and TrpOH-ir neurons in sections from rats exposed to A) CO, B) LL, C) HL conditions in Experiment 1. Black boxes indicate regions shown at higher magnification in insets in the lower left hand corner of each panel. Arrows indicate examples of c-Fos-ir cells (blue/black nuclear staining); white arrowheads indicate c-Fos-immunonegative/TrpOH-ir (serotonergic) neurons (brown/orange cytoplasmic staining), filled arrowheads indicate c-Fos-ir/TrpOH-ir (double-immunostained) neurons. Abbreviation: Aq, cerebral aqueduct; bv, blood vessel, characteristic of the DRVL region at this rostrocaudal level; mlf, medial longitudinal fasciculus. Scale bar, 100 μm, inset 25 μm.

From: Hale MW, Hay-Schmidt A, Mikkelsen JD, Poulsen B, Bouwknecht JA, Evans AK, Stamper CE, Shekhar A, Lowry CA. Exposure to an open-field arena increases c-Fos expression in a subpopulation of neurons in the dorsal raphe nucleus, including neurons projecting to the basolateral amygdaloid complex.
Neuroscience. 2008 Dec 10;157(4):733-48.

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Published customer image:
Sheep anti Rabbit tryptophan hydroxylase antibody used for the demonstration of immunoreactive neurons in rat brain by immunofluorescence on cryostat sections.
Image caption:
Double immunofluorescence photomicrograph illustrating a retrogradely labeled serotonergic neuron in the DRD region of the mid-rostrocaudal dorsal raphe nucleus (-8.18 mm Bregma) following unilateral intra-BL injection of CTb. A) Photomicrograph showing the mid-rostrocaudal dorsal raphe nucleus. White box in A indicates region shown at higher magnification in B. White box in B indicates region shown at higher magnification in C, D and E. TrpOH-ir (serotonergic) neurons appear red (C) while CTb-ir (retrogradely labeled) neuron appears green (D). Double TrpOH/CTb immunofluorescent neuron appears yellow (E). Scale bar (A) 200μm, (B) 80μm, (C), (D) & (E) 20μm.

From: Hale MW, Hay-Schmidt A, Mikkelsen JD, Poulsen B, Bouwknecht JA, Evans AK, Stamper CE, Shekhar A, Lowry CA. Exposure to an open-field arena increases c-Fos expression in a subpopulation of neurons in the dorsal raphe nucleus, including neurons projecting to the basolateral amygdaloid complex.
Neuroscience. 2008 Dec 10;157(4):733-48.

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Published customer image:
Sheep antiRabbit tryptophan hydroxylasae antibody used to ientify immunoreactive neurons by immunohistochemistry on cryostat sections.
Image caption:
Influence of M. vaccae or its derivatives on pulmonary cytokine mRNA expression, c-Fos expression in the nTS and c-Fos expression in serotonergic neurons in the dorsal raphe nucleus (DR). (a) Graphs illustrate mean levels, relative to β-actin, of pulmonary IL-1β (IL-1), TNF-α (TNF), and IL-6 mRNA expression 12 h, and 3, 6, 10, and 17 days following i.t. injection of Mv-NC in M. vaccae–preimmunized mice (•) relative to cytokine mRNA expression following i.t. injection of vehicle in M. vaccae–preimmunized mice (O) (12 h time point only), as well as, for comparison, cytokine mRNA expression at the same time points following i.t. injection of OVA-NC in OVA/alum-preimmunized mice (▼) or vehicle in OVA/alum-preimmunized mice (▽) (12 h time point only). (b) Bar graphs illustrate the mean number (±S.E.M.) of c-Fos-ir nuclei in the AP, SolDL, DRC, and DRI in experiment 1. (c) Photographs illustrate nuclear c-Fos immunostaining (blue–black) 12 h following i.t. injection of M. vaccae in M. vaccae–preimmunized mice in the AP and SolDL of the nTS (top two photographs) and serotonergic neurons in the DRC (middle two photographs) and DRI parts (bottom two photographs) of the DR. Tyrosine hydroxylase immunostaining (brown) was used to aid in identification of neuroanatomical subdivisions of the nTS. Tryptophan hydroxylase immunostaining (brown) was used to identify serotonergic neurons in the DRC and DRI. (⇒) c-Fos-immunonegative serotonergic neurons, (→) c-Fos-immunopositive serotonergic neurons. Small black boxes in (c) indicate regions shown at higher magnification in insets. Scale bar=100 μm (c) top row; (c) middle and bottom rows, 25 μm; (c) insets, 12.5 µm. Abbreviations: IL-1, interleukin-1β; (Mv), preimmunization with s.c. injections of heat-killed M. vaccae; Mv-NC, i.t. challenge with sonicated heat-killed Mv-NC; NC, i.t. challenge with NC. ' P≤0.05, compared with M. vaccae–preimmunized, vehicle-injected controls, Fisher's protected LSD test. For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.

From: Lowry CA, Hollis JH, de Vries A, Pan B, Brunet LR, Hunt JR, Paton JF, van Kampen E, Knight DM, Evans AK, Rook GA, Lightman SL. Identification of an immune-responsive mesolimbocortical serotonergic system: potential role in regulation of emotional behavior.
Neuroscience. 2007 May 11;146(2):756-72.

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Published customer image:
Sheep antiRabbit tryptophan hydroxylasae antibody used to ientify immunoreactive neurons by immunohistochemistry on cryostat sections.
Image caption:
Both i.t. Mv-NC and i.t. OVA-NC induced c-Fos expression in the nTS but only Mv-NC increased c-Fos expression in DRI serotonergic neurons. Immunostained products are the same as in Fig. 1. (a) Bar graphs illustrate the mean number (±S.E.M.) of c-Fos-ir nuclei in the AP and SolDL (left) and c-Fos-ir/tryptophan hydroxylase-ir neurons in the DRC and DRI (right) in experiment 2. (b, c) Photomicrographs illustrate c-Fos responses to Mv-NC in M. vaccae–preimmunized mice or to OVA-NC in OVA/alum-preimmunized mice in the SolDL and AP (b) or the DRI (c). (⇒) c-Fos-immunonegative serotonergic neurons, () c-Fos-immunopositive serotonergic neurons. Small black boxes indicate regions shown at higher magnification in insets. Scale bar=100 μm b; b (insets), 50 μm; c, 25 μm; c (insets) 12.5 μm. Abbreviations: (OVA), preimmunization with s.c. injections of OVA/alum; OVA-NC, i.t. challenge with OVA-NC. For additional abbreviations, see Fig. 1 legend. * P≤0.05, compared with the appropriate M. vaccae– or OVA/alum-preimmunized, vehicle-injected control group, Fisher's protected LSD test.

From: Lowry CA, Hollis JH, de Vries A, Pan B, Brunet LR, Hunt JR, Paton JF, van Kampen E, Knight DM, Evans AK, Rook GA, Lightman SL. Identification of an immune-responsive mesolimbocortical serotonergic system: potential role in regulation of emotional behavior.
Neuroscience. 2007 May 11;146(2):756-72.

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Tryptophan Hydroxylase Antibody gallery image 9

Published customer image:
Sheep anti Rabbit tryptophan hydroxylasae antibody used to ientify immunoreactive neurons by immunohistochemistry on cryostat sections.
Image caption:
Activation of bronchopulmonary afferent vagal pathways was not necessary for the effects of Mv-NC on DRI serotonergic neurons. Immunostained products are the same as in Fig. 1. (a) Bar graphs illustrate the mean number (±S.E.M.) of c-Fos-ir nuclei in the AP and SolDL (left) and c-Fos-ir/tryptophan hydroxylase-ir neurons in the DRC and DRI (right) in experiment 4. (b, c) Photomicrographs illustrate c-Fos responses to i.t. Mv-NC or s.c. M. vaccae in M. vaccae–preimmunized mice in the SolDL and AP (b) or the DRI (c) at the 12 h time point. For abbreviations, see Fig. 1 legend. (⇒) c-Fos-immunonegative serotonergic neurons, (→) c-Fos-immunopositive serotonergic neurons. Scale bar=100 μm b; b (insets), 50 μm; c, 25 μm; c (insets), 12.5 μm. Abbreviations: Mv, s.c. challenge with heat-killed M. vaccae; Sal, s.c. challenge with saline vehicle. For additional abbreviations, see Fig. 1 legend. * P≤0.05, compared with the appropriate M. vaccae–preimmunized, vehicle-injected control group, Fisher's protected LSD test.

From: Lowry CA, Hollis JH, de Vries A, Pan B, Brunet LR, Hunt JR, Paton JF, van Kampen E, Knight DM, Evans AK, Rook GA, Lightman SL. Identification of an immune-responsive mesolimbocortical serotonergic system: potential role in regulation of emotional behavior.
Neuroscience. 2007 May 11;146(2):756-72.

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  • Sheep anti Rabbit Tryptophan Hydroxylase
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    9260-2505C, WBdatasheet pdfdatasheet pdf25 µl
    9260-2505
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Sheep anti Rabbit tryptophan hydroxylase antibody recognises tryptophan hydroxylase, also known as Tryptophan 5-hydroxylase 1 or Tryptophan 5-monooxygenase 1. Tryptophan hydroxylase is a 444 amino acid ~55 kDa enzyme involved in the synthesis of the neurotransmitter serotonin.
    • Intended Use
    • Target Species
      Rabbit
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      Humanyes
      MammalsExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified Ig - liquid
    • Reconstitution
    • Preparation
      Purified Ig prepared by affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3), 50% Glycerol,
      0.01% Bovine Serum Albumin.
    • Immunogen
      Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      aromatic amino acid family metabolic process
      serotonin biosynthetic process
      iron ion binding
      oxidation-reduction process
      amino acid binding
      tryptophan 5-monooxygenase activity
    • UniProt
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
      9260-2505 has been used for immunohistochemistry on formaldehyde fixed tissue, it is useful for cell body but not fiber staining
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Tryptophan Hydroxylase Antibody Formats

    Formats Applications Sizes available
    Tryptophan Hydroxylase Antibody : Purified C, WB 25 µl
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunohistology - Frozen

              References

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