Thymocytes/Neutrophils/T Cells/Platelets Antibody | RPN3/57

Thymocytes/Neutrophils/T Cells/Platelets Antibody | RPN3/57 gallery image 1

Published customer image:
Muscle cross-sections from the non-exercised soleus muscle after 6w of EMS/E (A, B, D–G) and from the exercised side of the gastrocnemius muscle (C). The sections A–C are stained for demonstration of neutrophils/T-lymphocytes (mAb MCA805G). The framed region in (A) is in higher magnification in (B) and the parallel section to the framed region stained for H&E is in the inset (B). The figures show a large number of immuno-reactive cells (arrows) in the connective tissue (A–C). Stars show corresponding muscle fiber in (A) and (B). Figure D show infiltration of inflammatory cells in a necrotic fiber stained with H&E and figure E show the corresponding necrotic fiber (asterix) stained with mAb M0814 against CD68 (macrophages). Figure F show eosinophils (mAb MAB1087) infiltrating a muscle fiber (arrow) In (G), eosinophils (arrows) are stained with H&E in the extracellular matrix of another region of the muscle sample. (Original magnification; A ×100, B–E ×200, F and G x315).

From: Song Y, Forsgren S, Yu J, Lorentzon R, Stål PS (2012) Effects on Contralateral Muscles after Unilateral Electrical Muscle Stimulation and Exercise. PLoS ONE 7(12): e52230.

Thymocytes/Neutrophils/T Cells/Platelets Antibody | RPN3/57 gallery image 2

Published customer image:
Double-staining for tachykinin/white blood cell markers. Sections of soleus muscle tissue of the 6w group, non-exercised side (A-C) and section of gastrocnemius muscle tissue of the 1w group, exercised side (D,E). Double stainings for tachykinin (sc-14104) (green) and neutrophil/T-cell marker (MCA805G) (yellowish) are shown in (A) and (D,E). In (B), double staining for tachykinin (green) and macrophage marker (CD68) (yellowish) is shown and in (C), double staining for tachykinin and eosinophil marker (yellowish) is demonstrated. A white blood cell shows immunoreactivity for the neutrophil/T-cell marker (arrow) but does not show tachykinin-like immunoreactivity (A). There is on the other hand tachykinin-like immunoreactivity (green) in the smooth muscle of a vessel wall (asterisk); the sign (#) depicts debris material within the lumen of the blood vessel (A). There are unspecific reactions in the endothelium of the vessel wall (A). Note that tachykinin-like immunoreactivity (green) can be seen in the macrophage (arrowhead) in (B). There is a blood vessel, in which wall there is only tachykinin-like immunoreactivity (asterisk) (B). There is also tachykinin-like immunoreactivity in eosinophils (arrowheads) (C). Concerning (D,E): The occurrence of reactions for white blood cells after processing for MCA805G is shown in (D) (arrows). The combination of reactions for tachykinin/DAPI is shown in (E). The same cells as indicated in (D) are indicated in (E) (arrows). Note that the cells are not exhibiting tachykinin-like immunoreactivity. There is on the other hand tachykinin-like immunoreactivity in the vessel wall in (E) (asterisk). The reaction for MCA805G is masked in (E). Magnification x315.

From: Song, Y. et al. 2013 Bilateral increase in expression and concentration of tachykinin in a unilateral rabbit muscle overuse model that leads to myositis. BMC Musculoskelet Disord. 2013. 14:134.

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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA805GAC, Fdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Rabbit T Cells and Neutrophils antibody, clone RPN3/57 recognizes a subset of T cells (but not RL-5 cell line) and neutrophils by flow cytometry. Gives good staining of T-dependent areas in lymphoid tissue and also of infiltrating neutrophils by immunohistochemistry.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Rabbit Peritoneal Neutrophils.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from innunized Balb/c mice were fuzed with cells of the 653WMB plamacytoma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Thymocytes/Neutrophils/T Cells/Platelets Antibody Formats

    Formats Clone Applications Sizes available
    Thymocytes/Neutrophils/T Cells/Platelets Antibody : Purified RPN3/57 C, F 0.1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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