Phosphorylase B Antibody

//www.bio-rad-antibodies.com/rabbit-lapine-phosphorylase-b-antibody-7370-9808g.html
Phosphorylase B Antibody gallery image 1

Rabbit Phosphorylase B detected with Sheep anti Rabbit Phosphorylase B (7370-9808G)

Enlarge
  • Phosphorylase B Antibody thumbnail image 1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    7370-9808E, WBdatasheet pdfdatasheet pdf0.5 ml
    7370-9808
    7370-9808GEdatasheet pdfdatasheet pdf1 ml
    7370-9808G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • 7370-9808G recognises phosphorylase B, a catalytic protein with a predicted molecular weight of 125 kDa localized in the cytoplasm and cell membrane.

      In skeletal muscle, phosphorylase occurs in two forms, phosphorylase A and phosphorylase B. Phosphorylase A is the more active of the two forms and has a higher molecular weight. The two forms are interconvertable. Phosphorylase B catalyzes the phosphorylation of serine residues in various substrates including troponin I.

      7370-9808G shows high cross reactivity (>95%) with phosphorylase A in ELISA.
    • Intended Use
    • Target Species
      Rabbit
    • Product Form
      Serum - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      None present
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Native, from muscle
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 5.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      glucan 1,4-alpha-glucosidase activity
      calmodulin binding
      glycogen metabolic process
      plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/6001/2000
      Western Blotting1/10001/2000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Phosphorylase B Antibody Formats

    Formats Applications Sizes available
    Phosphorylase B Antibody : Purified E 1 ml
    Phosphorylase B Antibody : Serum E, WB 0.5 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Western Blotting

              References

              Write your review

              You may also be interested in...

              You May Also Be Interested In...