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MHC Class II DQ antibody | 2C4

Mouse anti Rabbit MHC Class II DQ

Product Type
Monoclonal Antibody
Clone
2C4
Isotype
IgG2a
Specificity
MHC Class II DQ

Product Code Applications Pack Size List Price Your Price Qty
MCA811GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.1 mg loader
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loader

Mouse anti Rabbit MHC class II monoclonal antibody, clone 2C4 recognizes the class II RLA-DQ molecule. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rabbits, this complex is referred to as the rabbit leukocyte antigen (RLA) region. There are 3 major MHC class II proteins encoded by the RLA which are RLA DP, RLA DQ and RLA DR.

Mouse anti rabbit MHC class II recognizes the RLA DQ molecule, expressed by antigen presenting cells, B cells and monocytes.

Mouse anti rabbit MHC class II monoclonal antibody, clone 2C4 is able to significantly inhibit the mixed lymphocyte reaction. Immunoprecipitation with the antibody yields three bands of molecular weights 28, 31 and 35 kDa.

Target Species
Rabbit
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Rabbit spleen cells
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the P3.X63. Ag8.653 mouse myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

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Source Reference

  1. Lobel, S.A. et al. (1984) The role of rabbit Ia molecules in immune functions as determined with the use of an anti-Ia monoclonal antibody.
    Immunology 51: 35-43.

References for MHC Class II DQ antibody

  1. Spieker-Polet, H. et al. (1990) Rabbit major histocompatibility complex. IV. Expression of major histocompatibility complex class II genes.
    J Immunogenet. 17 (1-2): 123-32.
  2. Wilkinson, J.M. et al. (1992) A cytotoxic rabbit T-cell line infected with a gamma-herpes virus which expresses CD8 and class II antigens.
    Immunology 77: 106-8.
  3. Idogawa, H. et al. (1997) Progression of articular destruction and the production of tumour necrosis factor-alpha in antigen-induced arthritis in rabbits.
    Scand J Immunol. 46: 572-80.
  4. Andersen, H.O. et al. (1999) Effect of cyclosporine on arterial balloon injury lesions in cholesterol-clamped rabbits: T lymphocyte-mediated immune responses not involved in balloon injury-induced neointimal proliferation.
    Arterioscler Thromb Vasc Biol. 19: 1687-94.
  5. Matsumura, T. et al. (1999) Suppression of atherosclerotic development in Watanabe heritable hyperlipidemic rabbits treated with an oral antiallergic drug, tranilast.
    Circulation 99 (7): 919-24.
  6. Alt, C. et al. (2009) Increased CCL2 expression and macrophage/monocyte migration during microbicide-induced vaginal irritation.
    Curr HIV Res. 7: 639-49.
  7. Yuan, T. et al. (2010) Chondrogenic differentiation and immunological properties of mesenchymal stem cells in collagen type I hydrogel.
    Biotechnol Prog. 26 (6): 1749-58.
  8. Shen, W. et al. (2013) Osteoarthritis prevention through meniscal regeneration induced by intra-articular injection of meniscus stem cells.
    Stem Cells Dev. 22 (14): 2071-82.
  9. View The Latest Product References
  10. Sun, F. et al. (2023) Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one-step.
    Bioeng Transl Med. 8 (4): e10534.

Flow Cytometry

MCA811GA

1610 166443

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