MHC Class I Monomorphic Antibody | 73-2

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MHC Class I Monomorphic Antibody | 73-2 gallery image 1

Staining of lapine peripheral blood lymphocytes with Mouse anti Rabbit MHC Class I Monomorphic (MCA810GA) followed by Goat anti Mouse IgG (H/L):FITC (STAR117F)

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  • MHC Class I Monomorphic Antibody | 73-2 thumbnail image 1
  • Mouse anti Rabbit MHC Class I Monomorphic
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  • Product Type
    Monoclonal Antibody
  • Clone
    73-2
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA810GAFdatasheet pdfdatasheet pdf0.1 mg
    MCA810GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rabbit MHC class I monomorphic, clone 73-2, recognizes the lapine homologue of the human major histocompatibility complex (MHC) class Ib, a protein expressed at varying levels on the cell surface of the majority of nucleated rabbit cells.

      MHC genes are found in all vertebrate species. The nomenclature varies for different species, often collectively named as leukocyte antigens and prefixed by the species or species group, e.g. human leukocyte antigen (HLA), bovine leukocyte antigen (BoLA) etc. Rabbit major histocompatibility complex molecules are generally referred to as MHC, however some sources have referenced them as Rabbit leukocyte antigens (RLA). MHC molecules comprise a vital component of adaptive immunity, MHC class I molecules are glycoproteins that function in the presentation of antigen fragments from antigen processing cells to cytotoxic T cells.

      Clone 73-2 is specific for the rabbit MHC class Ib and provides a useful flow cytometry marker of rabbit nucleated cells.
    • Intended Use
    • Target Species
      Rabbit
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Rabbit T cells
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1:501:200

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional MHC Class I Monomorphic Antibody Formats

    Formats Clone Applications Sizes available
    MHC Class I Monomorphic Antibody : Purified 73-2 F 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed)STAR117D405GA0.1 mgF, IF
      STAR117D405GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Rabbit CD8:FITCMCA1576F0.1 mgF
          MCA1576F
          Mouse anti Rabbit IgM (B Cell Marker):FITCMCA812F0.1 mgF
          MCA812F
          Mouse anti Rabbit CD4:FITCMCA799F100 TestsF
          MCA799F
          Mouse anti Rabbit CD45MCA808GA0.1 mgC, F, IF
          MCA808GA
          Mouse anti Rabbit IgM (B Cell Marker):RPEMCA812PE100 TestsF
          MCA812PE

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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