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IgG Antibody

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Detection of Rabbit anti Human MAP1LC3A/B (AHP2167) in a Western blot of HeLa cell lysate using Sheep anti Rabbit IgG:HRP (STAR54)

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Detection of Rabbit anti Human MAML3 (AHP2218) in an immunohistochemical analysis of formalin fixed paraffin embedded mouse intestine using Sheep anti Rabbit IgG:HRP (STAR54)

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Published customer image:
Horseragish peroxidase conjugated Sheep anti Rabbit IgG antibody (STAR54) used for the detection of bound Rabbit anti Rat IL-1&beta antibody (AAR15G) on formalin fixed, paraffin embedded rat brain sections by immunohistochemistry.
Image caption:
Neuronal expression of IL-1ß in the right hemisphere exposed to lipopolysaccharide (LPS) + hypoxia-ischemia (HI). Mean ± standard error of the mean (SEM) (pg/mg) of IL-1ß (A) and TNF-a (B). Titrations were done by ELISA on total protein extracts from the right cerebral hemisphere exposed to LPS plus HI for 4 h to 24 h compared to control (Ctl). (C) Immunofluorescent staining of neuronal nuclei (NeuN) + IL-1ß + cells in the fronto-parietal neocortex (D). Mean ± SEM (%) of neural cells expressing Il-1ß. (E) Immunohistochemical staining of IL-1ß expression on neurons (arrows) from fronto-parietal neocortex and caudate putamen at 4 h post-LPS + HI compared to Ctl. Scale bar = 50 µm (4 to 6 pups per experimental condition). Analysis of variance with Newman-Keuls post-test; *P = 0.05, **P = 0.01. h hour, F-P Fronto-parietal, WM White matter, CPu Caudate putamen, Iba1 Ionized calcium-binding adapter molecule 1, GFAP Glial fibrillary acidic protein.

From: Savard A, Lavoie K, Brochu ME, Grbic D, Lepage M, Gris D, Sebire G. Involvement of neuronal IL-1ß in acquired brain lesions in a rat model of neonatal encephalopathy.
J Neuroinflammation. 2013 Sep 5;10:110.

IgG Antibody gallery image 4

Detection of Rabbit anti Human SP1 (AHP850) in a Western blot analysis of a HeLa cell nuclear extract using Sheep anti Rabbit IgG:HRP (STAR54)

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  • Sheep anti Rabbit IgG:HRP (Multi Adsorbed)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR54C, E, WBdatasheet pdfdatasheet pdf1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Sheep anti Rabbit IgG antibody conjugated to HRP recognizes rabbit IgG as demonstrated by ELISA and double diffusion.

      Cross-reactivity against human, mouse, rat and bovine immunoglobulins is reduced by solid phase adsorption.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG fraction conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to rabbit IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.05% Proclin™ 300
      0.002% Methylisothiazolone
      0.002% Bromonitrodioxane
      1% Bovine Serum Albumin
    • Immunogen
      Purified rabbit IgG from normal rabbit serum.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/501/100
      Western Blotting1/10000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : HRP C, E, WB 1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TMB Signal+BUF054A100 mlE
          TMB CoreBUF056A100 mlE
          TMB Core PrestainedBUF057A100 mlE
          TMB Core+BUF062A100 mlE

          Recommended Positive Controls

            Histology Controls

                • Product Images

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