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Horseragish peroxidase conjugated Sheep anti Rabbit IgG antibody (STAR54) used for the detection of bound Rabbit anti Rat IL-1&beta antibody (AAR15G) on formalin fixed, paraffin embedded rat brain sections by immunohistochemistry.
Neuronal expression of IL-1ß in the right hemisphere exposed to lipopolysaccharide (LPS) + hypoxia-ischemia (HI). Mean ± standard error of the mean (SEM) (pg/mg) of IL-1ß (A) and TNF-a (B). Titrations were done by ELISA on total protein extracts from the right cerebral hemisphere exposed to LPS plus HI for 4 h to 24 h compared to control (Ctl). (C) Immunofluorescent staining of neuronal nuclei (NeuN) + IL-1ß + cells in the fronto-parietal neocortex (D). Mean ± SEM (%) of neural cells expressing Il-1ß. (E) Immunohistochemical staining of IL-1ß expression on neurons (arrows) from fronto-parietal neocortex and caudate putamen at 4 h post-LPS + HI compared to Ctl. Scale bar = 50 µm (4 to 6 pups per experimental condition). Analysis of variance with Newman-Keuls post-test; *P = 0.05, **P = 0.01. h hour, F-P Fronto-parietal, WM White matter, CPu Caudate putamen, Iba1 Ionized calcium-binding adapter molecule 1, GFAP Glial fibrillary acidic protein.
From: Savard A, Lavoie K, Brochu ME, Grbic D, Lepage M, Gris D, Sebire G. Involvement of neuronal IL-1ß in acquired brain lesions in a rat model of neonatal encephalopathy.
J Neuroinflammation. 2013 Sep 5;10:110.