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Goat anti Rabbit IgG antibody (403001) used as a capture antibody in a sandwich ELISA for the evaluation of total rabbit IgG in serum and vaginal lavage samples.
PRO 2000 co-application induces increased systemic and mucosal antibody responses in rabbits.
New Zealand White rabbits (n = 6) received six vaginal immunizations using 50 mg antigen per dose in the presence or absence of 1% PRO 2000. (A) Time course of rabbit serum IgG endpoint titres. (B) Endpoint IgG titres of week 12 vaginal lavage samples. Red symbols represent data from animals immunized with gp140 + PRO 2000. Pre-immune indicates samples collected prior to the first immunization; gp140 + PRO indicates combined gp140 and PRO 2000. Titre data were log-transformed and are shown as mean ± SEM. The grey dashed line indicates the ELISA detection limit. * p<0.05; ** p<0.01.
From: Wegmann F, Krashias G, Lühn K, Laamanen K, Vieira S, Jeffs SA, et al. (2011)
A Novel Strategy for Inducing Enhanced Mucosal HIV-1 Antibody Responses in an Anti-Inflammatory Environment.
PLoS ONE 6(1): e15861.