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Mouse anti Rabbit CD45 antibody, clone L12/201 used for the evaluation ofleukocyte infiltrates in conjunctival associated lymphoid tissue by flow cytometry and immunofluorescence microscopy.
Conjunctival imprints evaluated by flow cytometry for CD45-positive cells after multiple instillations at H4, and assessed after cytospin centrifugation. The inflammatory marker clearly showed the highest expression in BAC-containing eye drops (A). PBS- and LCEm- instilled groups had only a basal level of expression. CD45-positive cells (green) after instillations of BAC+latanoprost (B) were viewed after propidium iodide staining (red) and cytospin centrifugation. The scale bar indicates 50 µm. The asterisk indicates a p<0.05 compared with BAC+latanoprost-instilled group. The double asterisk indicates a p<0.02 compared with BAC+latanoprost-instilled group.
From: Liang H, Baudouin C, Faure MO, Lambert G, Brignole-Baudouin F. Comparison of the ocular tolerability of a latanoprost cationic emulsion versus conventional formulations of prostaglandins: an in vivo toxicity assay.
Mol Vis. 2009 Aug 25;15:1690-9.