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Mouse anti Rabbit CD44 antibody used for the evaluation of CD44 expression on CD8 expressing cells harveted from different lymphoid and non-lymphoid organs by flow cytometry.
CD25 and CD44 expression in CD8+ T cells isolated from lymphoid and non-lymphoid organs during WD-MCF in rabbits. At time of euthanasia, analysis by multi-colour flow cytometry was conducted on PBMC and mononuclear leukocytes isolated from pLN, spleen, liver, lung and kidney of mock-infected or AlHV-1-infected rabbits developing WD-MCF. (A) Half-offset histograms of CD25 or CD44 expression by CD4+ (left panel) or CD8+ cells (right panel) in mononuclear leukocytes isolated from pLN of one representative rabbit of each group. Data were obtained by triple-staining of CD4 or CD8 with isotype control (grey histograms) or CD25 and CD44 (blue histograms) as described in Methods. (B) Mean fluorescence intensities of CD25 and CD44 in CD4+ (left panel) or CD8+ cells (right panel) obtained by multi-colour flow cytometry analysis. The percentages of CD4+ or CD8+ cells in the kidney of mock-infected animals were below 3% and were therefore not plotted. Data are representative of two independent experiments (n = 3). Bars show means ± SEM. *p < 0.05, **p < 0.01.
From: Dewals BG, Vanderplasschen A.
Malignant catarrhal fever induced by Alcelaphineherpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues.
Vet Res. 2011 Aug 22;42:95.