CD44 Antibody | W4/86

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CD44 Antibody | W4/86 gallery image 1

Rabbit peripheral blood granulocytes stained with Mouse anti Rabbit CD44 (MCA806GA) followed by Goat anti Mouse IgG:FITC (STAR117F)

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CD44 Antibody | W4/86 gallery image 2

Published customer image:
Mouse anti Rabbit CD44 antibody used for the evaluation of CD44 expression on CD8 expressing cells harveted from different lymphoid and non-lymphoid organs by flow cytometry.
Image caption:
CD25 and CD44 expression in CD8+ T cells isolated from lymphoid and non-lymphoid organs during WD-MCF in rabbits. At time of euthanasia, analysis by multi-colour flow cytometry was conducted on PBMC and mononuclear leukocytes isolated from pLN, spleen, liver, lung and kidney of mock-infected or AlHV-1-infected rabbits developing WD-MCF. (A) Half-offset histograms of CD25 or CD44 expression by CD4+ (left panel) or CD8+ cells (right panel) in mononuclear leukocytes isolated from pLN of one representative rabbit of each group. Data were obtained by triple-staining of CD4 or CD8 with isotype control (grey histograms) or CD25 and CD44 (blue histograms) as described in Methods. (B) Mean fluorescence intensities of CD25 and CD44 in CD4+ (left panel) or CD8+ cells (right panel) obtained by multi-colour flow cytometry analysis. The percentages of CD4+ or CD8+ cells in the kidney of mock-infected animals were below 3% and were therefore not plotted. Data are representative of two independent experiments (n = 3). Bars show means ± SEM. *p < 0.05, **p < 0.01.

From: Dewals BG, Vanderplasschen A.
Malignant catarrhal fever induced by Alcelaphineherpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues.
Vet Res. 2011 Aug 22;42:95.

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  • Mouse anti Rabbit CD44
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  • Product Type
    Monoclonal Antibody
  • Clone
    W4/86
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA806GAC, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA806GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rabbit CD44 antibody, clone W4/86 recognizes the rabbit CD44 cell surface antigen, a ~95 kDa glycoprotein expressed by all leucocytes. In immunohistochemical staining the antibody labels the medullary area strongly and the cortical area weakly.

      Mouse anti Rabbit CD44 antibody, clone W4/86 has been reported as being suitable for use in Western blotting under non-reducing conditions.
    • Intended Use
    • Target Species
      Rabbit
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      RL-5 T cell line glycoproteins.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from an immunized mouse were fused with cells of the mouse P3.X63 Ag8 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/200
      Immunohistology - Frozen
      Immunoprecipitation

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD44 Antibody Formats

    Formats Clone Applications Sizes available
    CD44 Antibody : Purified W4/86 C, F, IP 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

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