Native Rabbit ACE is Rabbit angiotensin converting enzyme purified from lung. CAS number: 9015-82-1 EC number: 220.127.116.11
Purified protein from rabbit lung - liquid
4.5 U/mg protein. One unit will produce 1.0 micromole of hippuric acid from hippuryl-His-Leu per minute in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37ºC. EDTA can be used as a strong inhibitor of this enzyme. This can be reversed by dialysis and reactivation with metal salts. The Km for this enzyme is 2.6 mM using hippuryl-L-histidyl-L-leucine as the substrate.
Approx. Protein Concentrations
Reported as approximately 129 kDa (sedimentation equilibrium with and without 6 M guanidine hydrochloride) and approximately 140 kDa (SDS gel electrophoresis and by gel filtration).
Reagents In The Kit
Preparing The Antibody
Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.