ACE

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  • Native Rabbit ACE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    0100-0313FNdatasheet pdfdatasheet pdf0.25 Units
    0100-0313
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Native Rabbit ACE is Rabbit angiotensin converting enzyme purified from lung.
      CAS number: 9015-82-1
      EC number: 3.4.15.1
    • Intended Use
    • Target Species
      Rabbit
    • Product Form
      Purified protein from rabbit lung - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      None present.
    • Activity
      4.5 U/mg protein.

      One unit will produce 1.0 micromole of hippuric acid from hippuryl-His-Leu per minute in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37ºC.

      EDTA can be used as a strong inhibitor of this enzyme. This can be reversed by dialysis and reactivation with metal salts.

      The Km for this enzyme is 2.6 mM using hippuryl-L-histidyl-L-leucine as the substrate.
    • Purity
    • Approx. Protein Concentrations
      10 Units/ml
    • Molecular Weight
      Reported as approximately 129 kDa (sedimentation equilibrium with and without 6 M guanidine hydrochloride) and approximately 140 kDa (SDS gel electrophoresis and by gel filtration).
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      integral to membrane
      carboxypeptidase activity
      proteolysis
      metallopeptidase activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional ACE Formats

    Formats Applications Sizes available
    ACE : Purified FN 0.25 Units
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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