Goat anti Rabbit pyruvate kinase antibody recognizes the enzyme pyruvate kinase isolated from rabbit muscle Pyruvate kinase is an enzyme invloved in glycolysis that catalyzes the transfer of phosphate from phosphoenol pyruvate (PEP) to ADP yielding ATP and pyruvate. Pyruvate can then be converted to Acetyl CoA and enter the TCA cycle. At least three molecular forms with pyruvate kinase activity are known. PK1, the red cell form, is deficient in a type of hemolytic anemia. PK2 is found in kidney. PK3 is found in leukocytes, muscle, platelets, and brain but not in red cells or kidney.
Assay by IEP gives a single precipitin arc against anti-goat serum as well as partially purified rabbit muscle pyruvate kinase. Goat anti Rabbit pyruvate kinase antibody has been reported to react with all forms of pyruvate kinase.
Ig fraction - liquid
Ig fraction prepared by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Sodium Azide (NaN3)
Pyruvate kinase from rabbit muscle.
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.