Pyruvate Kinase Antibody

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  • Goat anti Pyruvate Kinase
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    7894-9988E, WBdatasheet pdfdatasheet pdf1 ml
    7894-9988
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Pyruvate kinase is an enzyme invloved in glycolysis that catalyzes the transfer of phosphate from phosphoenol pyruvate (PEP) to ADP yielding ATP and pyruvate. Pyruvate can then be converted to Acetyl CoA and enter the TCA cycle. At least three molecular forms with pyruvate kinase activity are known. PK1, the red cell form, is deficient in a type of hemolytic anemia. PK2 is found in kidney. PK3 is found in leukocytes, muscle, platelets, and brain but not in red cells or kidney.

      Assay by IEP gives a single precipitin arc against anti-goat serum as well as partially purified rabbit muscle pyruvate kinase. This antibody has been reported to react with all forms of pyruvate kinase.
    • Intended Use
    • Product Form
      Ig fraction - liquid
    • Reconstitution
    • Preparation
      Ig fraction prepared by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Pyruvate kinase from rabbit muscle.
    • Purity
    • Approx. Protein Concentrations
      10.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Pyruvate Kinase Antibody Formats

    Formats Applications Sizes available
    Pyruvate Kinase Antibody : Purified E, WB 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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