PUREBLU™ Hoechst 33342

PUREBLU™ Hoechst 33342 gallery image 1

Paraffin section of human colon adenocarcinoma (HIER citrate pH6 antigen retrieval) blocked with 10% FCS and stained with mouse anti human cytokeratin 18 (MCA1864H) and detected with goat anti mouse IgG:A488 (STAR117D488GA). The tissue was also counterstained with PureBlu Hoechst 33342 (135-1304)

  • PUREBLU™ Hoechst 33342 thumbnail image 1
  • PUREBLU™ Hoechst 33342
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  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    1351304F, IFdatasheet pdfdatasheet pdf280 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Hoechst 33342 is a cell-permeable fluorescent compound that is able to stain the DNA of eukaryotic and prokaryotic cells by binding with high affinity to the minor groove of AT-rich DNA sequences. When Hoechst 33342 is bound to DNA and excited by an ultraviolet light source, blue fluoresecent emission can be detected with a maximum emission at 461 nm. PureBlu Hoescht 33342 has a characteristic Stokes shift of approximately 100 nm, which makes this dye an optimal choice when a good spectral separation is required. PureBlu Hoescht 33342 is compatible with fixed and unfixed cells. It exhibits high permeability for live cell membranes and is optimal for live cell DNA staining.
    • Intended Use
    • Product Form
    • Reconstitution
      1. Add 500 μl of de-ionized water to one tube of lyophilized PureBlu Hoechst 33342 Dye, then vortex briefly to make the 100x stock solution (1.1 μg/ml [2 μM]).

      2. Dilute the stock solution 1:100 with growth media (for live cells) or 1x phosphate buffered saline (for fixed cells) to make the 1x staining solution.
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Molecular Weight
    • Reagents In The Kit
      5 vials, 56 μg each, Hoechst 33342 nuclear staining dye powder
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Prior to reconstitution store at -20oC
      After reconstitution store at -20oC or +4oC
      This product is photosensitive and should be protected from light. PureBlu™ Hoechst 33342 is stable for 12 months from date of reconstitution if stored at 20oC or 6 months at 4oC.
    • Shelf Life
      Please see label for expiry date.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/100

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      Staining of Live Cells

      1. Grow cells of interest under conditions specific for the cell type.

      2. Replace growth media with 1x staining solution (diluted in fresh growth media) and incubate at 37oC for 15 minutes.

      3. Rinse cells with 1x phosphate buffered saline (prewarmed to 37oC).

      4. Aspirate phosphate buffered saline and add fresh growth media to cells (prewarmed to 37oC).

      5. Image cells.

      Staining of Fixed Cells

      1. Grow cells of interest under conditions specific for the cell type.

      2. Rinse cells with 1x phosphate buffered saline.

      3. Fix cells with 4% formaldehyde at room temperature for 10 minutes.

      4. Optional: Rinse cells with 1x phosphate buffered saline and permeabilize them with 0.1% Triton X-100 in 1x phosphate buffered saline at room temperature for 5 minutes.

      5. Rinse cells with 1x phosphate buffered saline.

      6. Stain with 1x staining solution (diluted with phosphate buffered saline) at room temperature for 15 minutes.

      7. Rinse cells with 1x phosphate buffered saline.

      8. Optional: Remove phosphate buffered saline and mount cells in antifade-mounting media.

      9. Image cells.

      Triton is trademark of Dow Chemical Company

    Additional PUREBLU™ Hoechst 33342 Formats

    Formats Applications Sizes available
    PUREBLU™ Hoechst 33342 : Reagent F, IF 280 µg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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