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Mouse anti Plasmodium falciparum MSP1 antibody, clone PEM-1 recognizes the merozoite surface protein 1 (MSP1) of Plasmodium falciparum, a protozoan parasite that causes malaria in humans. Malaria caused by P. falciparum has high mortality rates and is the most common in sub-Saharan Africa. The parasite is transmitted by Anopheles mosquitoes. Merozoites are the result of asexual reproduction of the parasite in the hepatocytes. They are released from hepatocytes and infected erythrocytes to infect other erythrocytes. MSP1 is the most abundant protein on the surface of merozoites and is involved in parasite invasion of the erythrocyte. It has long been considered a promising candidate for a vaccine against malaria. Some studies have shown that naturally acquired antibodies to a cleavage product of MSP1 can be linked to a reduced risk of clinical malaria.
Intended Use
Target Species
Protozoan
Product Form
Purified IgG - liquid
Reconstitution
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers
0.09%
Sodium Azide (NaN3)
Purity
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Reagents In The Kit
Preparing The Antibody
Test Principle
Buffer Solution
Phosphate buffered saline
Storage
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Acknowledgements
Regulatory
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name
Yes
No
Min Dilution
Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.