L-LACTATE Dehydrogenase Antibody | 13980

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L-LACTATE Dehydrogenase Antibody | 13980 gallery image 1

Recombinant Plasmodium vivax LDH detected in a sandwich ELISA using Human anti Plasmodium vivax LDH (HCA156) as the capture reagent and Human anti Plasmosium vivax LDH:HRP (HCA155P) as the detection reagent

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L-LACTATE Dehydrogenase Antibody | 13980 gallery image 2

Cross reactivity testing of Human anti Plasmodium vivax LDH (HCA156 and HCA155P) on Plasmodium falciparum LDH

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  • Human anti Plasmodium vivax LDH
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  • Product Type
    Monoclonal Antibody
  • Clone
    13980
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA156Edatasheet pdfdatasheet pdf0.1 mg
    HCA156
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • HCA156 recognises LDH (L-Lactose Dehydrogenase) from the Malaria causing protozoan species Plasmodium vivax. It was shown to cross-react with Plasmodium falciparum LDH.

      Clone 13980 was selected by phage display under heat stress conditions. The Tm value for thermal denaturation of the antibody was measured by CD spectroscopy and has been found to be 70 °C.

      HCA156 is designed for use as a capture antibody with HCA155P as the detection antibody in sandwich assay development.
    • Intended Use
    • Target Species
      Protozoan
    • Product Form
      Human IgG1 antibody selected from the HuCAL ® phage display library and expressed in a human cell line. This product is supplied as a liquid.
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Recombinant LDH fusion protein from Plasmodium vivax
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      oxidation-reduction process
      glycolysis
      L-lactate dehydrogenase activity
    • UniProt
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
      This product is suitable for use in indirect ELISA applications. This product may be used as a capture reagent in a sandwich ELISA together with MCA155P as the detection reagent.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional L-LACTATE Dehydrogenase Antibody Formats

    Formats Clone Applications Sizes available
    L-LACTATE Dehydrogenase Antibody : Purified 13980 E 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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