Sandwich assay with electro-chemiluminescence detection using the MSD Sector Imager instrument. Human anti plasmodium falciparum HRPII (HCA160) was used as the coating antibody. The antigen (recombinant HRPII from plasmodium falciparum) was titrated using the indicated concentrations. Detection was performed with ECL-labeled Human anti plasmodium falciparumHRPII (HCA159) antibody
Human anti plasmodium falciparum HRPII antibody, clone AbD14964 recognizes HRPII (histidine rich protein II) from the Malaria causing protozoan species Plasmodium falciparum .
Clone AbD14964 was selected by phage display under heat stress conditions. The Tm value for thermal denaturation of the antibody was measured by CD spectroscopy and has been found to be 70 °C. When conjugated this clone can be used as a detection antibody with HCA160 as the capture antibody in sandwich assay.
Human IgG1 antibody (kappa light chain) selected from the HuCAL ® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
Purified IgG prepared by affinity chromatography on Protein A
Recombinant HRPII fusion protein from Plasmodium falciparum
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC. DO NOT FREEZE. This product should be stored undiluted.
12 months from date of despatch
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.