Proteus IMAC mini/midi spin column kit

The Proteus mini kit contains:

  • 24 x Proteus spin column plugs containing Ni-IDA agarose resin.
  • 24 x Proteus spin columns (0.65 ml capacity in a fixed angle rotor).
  • 48 x 2.2 ml centrifuge tubes.
  • 24 x 10 kDa MWCO ultrafiltration spinners.
  • 1 x 250 ml 5 x PBS buffer (buffer A) bottle.
  • 1 x 150 ml 1 x imidazole buffer (buffer B) bottle.
  • Plug insertion tool. • Comprehensive handbook.
  • Ultrafiltration spinner instruction sheet.
  • Laminated protocol card.

NB. PUR023 contains the above, but without ultrafiltration spinners. PUR024 is a bulk spin column pack containing only Proteus spin column plugs, Proteus spin column barrels, and microcentrifuge tubes and the insertion tool.

Proteus IMAC mini kit contents

Proteus IMAC midi spin column kit

  • 8 x Proteus spin column plugs containing Ni-IDA agarose resin.
  • 8 x Proteus spin columns (20 ml capacity in a swing bucket rotor).
  • 12 x 50 ml centrifuge tubes. • 8 x 10 kDa MWCO ultrafiltration spinners.
  • 1 x 250 ml 5 x PBS buffer (buffer A) bottle.
  • 1 x 150 ml 1 x imidazole buffer (buffer B) bottle.
  • Plug insertion tool. • Comprehensive handbook.
  • Ultrafiltration spinner instruction sheet.
  • Laminated protocol card.

NB. PUR019 contains the above, but without ultrafiltration spinners. PUR020 is a bulk spin column pack containing only Proteus spin column plugs, Proteus spin column barrels, and microcentrifuge tubes and the insertion tool.

Proteus IMAC midi kit contents

Additional equipment recommended:

  • Syringe-end filters: 0.2 µm and 1.2 µm for clarification.
  • Quartz cuvettes for UV absorbance measurements.
  • UV/VIS spectrophotometer.
  • Pasteur pipettes and micro-pipettes.
  • Marker pen and test tube rack.
  • A bench-top centrifuge with swing bucket rotor that can accommodate 50 ml centrifuge tubes.
  • A microfuge that can accommodate 2.2 ml microcentrifuge tubes.

N.B. (For the Midi spin column only) The preferred rotor is a swing bucket rotor. For optimal performance with a fixed angle rotor, ensure that the orientation of the spin column in the rotor is the same for sample binding, washing and elution steps.

Storage conditions:

Remove the Proteus IMAC plug box from the kit and store it at 2-8 °C. There is no need to place the rest of the kit in a fridge or cold room. All buffers, for example, contain 0.1 % sodium azide and can be stored at room temperature. Do not freeze the resin plugs or store them at room temperature. Freezing the suspension will damage the agarose beads. The resin is pre-swollen and de-fined. It is transported in 20 % ethanol. Proteus spin columns are stable for up to 2 years at 2-8 °C from the date of manufacture. The expiry date is recorded clearly on the outside of the box.

Disclaimer:

This product is for research use only and is not intended for use in clinical diagnosis. No claims beyond replacement of unacceptable material or refund of purchase price shall be allowed.

Specifications:

Supporting Proteus matrix Covalently coupled to agarose resin
Charged metal ion Ni2+
Max sample volume

0.65 ml (Mini, Fixed angle rotor) 

20 ml (Midi, Swing bucket rotor)

Resin bed volume

0.23 ml (Mini)

1.6 ml (Midi)

Bead size range 45-165 µm
Recommended working pH pH 2.0-12.0
Typical number of uses per plugs

2 (Mini)

2 (Midi)

Typical binding capacity 1 mg His-tagged recombinant protein (Mini) 20 mg His-tagged recombinant protein (Midi)
Chemical stability High
Endotoxin levels Unknown
Solubility in water Insoluble
Plastic construction Polypropylene
Colour coded end-caps Black