Leukemia Inhibitory Factor | N/A

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Recombinant Rat Leukemia Inhibitory Factor

Product Type
Recombinant Protein
Clone
N/A
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
OBT1523 E FN 5 µg
Recombinant Rat Leukemia Inhibitory Factor is an Escherichia coli-derived recombinant protein corresponding to full-length mature rat leukemia inhibitory factor (LIF), a secreted peptide with the capability to induce terminal differentiation in leukemic cells.

Product Details

Target Species
Rat
Product Form
Recombinant Protein - liquid
Preparation
Recombinant rat LIF is expresssed as a GST-fusion protein, cleaved from GST via thrombin and purified by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
1mM DTT
10% Glycerol
0.02% Tween 20
Activity
>1 x 108 units/mg.

50 units is the amount required to induce differentiation in 50% of M1 colonies in 1ml agar cultures.
Purity
>85% by HPLC and SDS-PAGE analyses.
Approx. Protein Concentrations
Total protein concentration 10.0ug/ml
Molecular Weight
19.9 kDa (183 amino acid sequence)
Endotoxin Level
<0.1ng/ug

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P17777 Related reagents
Entrez Gene
Lif Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005146 leukemia inhibitory factor receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0010976 positive regulation of neuron projection development
GO:0016525 negative regulation of angiogenesis
GO:0031100 organ regeneration
GO:0048708 astrocyte differentiation
GO:0051461 positive regulation of adrenocorticotropin secretion
GO:0060041 retina development in camera-type eye
GO:0060290 transdifferentiation
Regulatory
For research purposes only

Applications of Leukemia Inhibitory Factor

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays 1/1000 1/10000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.