IL-6

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Recombinant Rat Interleukin-6

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PRP21 E FN WB 10 µg
Recombinant rat IL-6 is a 21.7 kDa peptide containing 188 residues corresponding to full-length mature rat interleukin-6.

IL-6 is a pleiotropic cytokine involved in the regulation of immune and inflammatory responses.

Product Details

Target Species
Rat
Product Form
Purified recombinant protein expressed in E. coli - lyophilised.
Reconstitution
Reconstitute with 0.1 ml distilled water. For extended storage the addition of 0.1% BSA is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Buffer Solution
TRIS buffered saline
0.1% SDS
Preservative Stabilisers
None present
Activity
ED50 of < 0.01ng/ml when assayed by proliferation of IL-6 dependent murine 7TD1 cells. This corresponds to a specific activity of 1 x 108 units/mg.
Purity
>98% by SDS PAGE and HPLC analysis.
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Protein Molecular Weight
21.7kD (188 Amino acids)
Endotoxin Level
<0.1ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P20607 Related reagents
Entrez Gene
Il6 Related reagents
GO Terms
GO:0006953 acute-phase response
GO:0005125 cytokine activity
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0045454 cell redox homeostasis
GO:0006469 negative regulation of protein kinase activity
GO:0009612 response to mechanical stimulus
GO:0006955 immune response
GO:0007568 aging
GO:0008083 growth factor activity
GO:0008285 negative regulation of cell proliferation
GO:0008360 regulation of cell shape
GO:0042493 response to drug
GO:0009408 response to heat
GO:0009409 response to cold
GO:0046677 response to antibiotic
GO:0031000 response to caffeine
GO:0031667 response to nutrient levels
GO:0032496 response to lipopolysaccharide
GO:0032868 response to insulin stimulus
GO:0033160 positive regulation of protein import into nucleus, translocation
GO:0034097 response to cytokine stimulus
GO:0043154 negative regulation of caspase activity
GO:0043200 response to amino acid stimulus
GO:0043526 neuroprotection
GO:0045188 regulation of circadian sleep/wake cycle, non-REM sleep
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045666 positive regulation of neuron differentiation
GO:0045721 negative regulation of gluconeogenesis
GO:0045740 positive regulation of DNA replication
GO:0046427 positive regulation of JAK-STAT cascade
GO:0046849 bone remodeling
GO:0048635 negative regulation of muscle organ development
GO:0050710 negative regulation of cytokine secretion
GO:0051592 response to calcium ion
GO:0051602 response to electrical stimulus
GO:0051789 response to protein stimulus
GO:0051971 positive regulation of transmission of nerve impulse
GO:0070374 positive regulation of ERK1 and ERK2 cascade
Regulatory
For research purposes only

Applications of IL-6

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 0.01 10ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.
ELISA
Recombinant rat IL-6 may be used as a standard for ELISA applications with either anti Rat IL-6: purified (AAR35) or anti Rat IL-6: biotin conjugate (AAR17B).
Western Blotting
Recombinant Rat IL-6 may be used as the positive control for western blotting applications with either anti Rat IL-6: purified (AAR35) or anti Rat IL-6: biotin conjugate (AAR17B).