IL-10

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Recombinant Rat Interleukin-10

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PRP29 E FN WB 10 µg
Rat IL-10 is a homodimeric protein, each 18.7 kDa subunit is 160 amino acid residues in length. IL-10 is a potent inhibitor of several cytokines including IFN gamma, IL-2 and TNF.

Interleukin-10 (IL-10) is produced primarily by activated T lymphocytes.

Product Details

Target Species
Rat
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 10ul water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For extended storage te addtion of 0.1% BSA is recommended,
Preparation
Recombinant protein prepared from E.Coli
Buffer Solution
Sodium Phosphate
Preservative Stabilisers
None present
Activity
Determined by the dose-dependent inhibition of antigen-specific T-cell proliferation is ≤ 10ng/ml
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
1.0 mg/ml
Protein Molecular Weight
18.7kD (160 amino acid sequence)
Endotoxin Level
<0.1ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P29456 Related reagents
Entrez Gene
Il10 Related reagents
GO Terms
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0002904 positive regulation of B cell apoptosis
GO:0005125 cytokine activity
GO:0005141 interleukin-10 receptor binding
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0042493 response to drug
GO:0014823 response to activity
GO:0030889 negative regulation of B cell proliferation
GO:0032496 response to lipopolysaccharide
GO:0032715 negative regulation of interleukin-6 production
GO:0032800 receptor biosynthetic process
GO:0032868 response to insulin stimulus
GO:0034465 response to carbon monoxide
GO:0042536 negative regulation of tumor necrosis factor biosynthetic process
GO:0043066 negative regulation of apoptosis
GO:0043193 positive regulation of gene-specific transcription
GO:0045019 negative regulation of nitric oxide biosynthetic process
GO:0050715 positive regulation of cytokine secretion
GO:0051045 negative regulation of membrane protein ectodomain proteolysis
GO:0051091 positive regulation of transcription factor activity
GO:0051384 response to glucocorticoid stimulus
GO:0051930 regulation of sensory perception of pain
GO:0071392 cellular response to estradiol stimulus
Regulatory
For research purposes only

Applications of IL-10

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 0.1 10.0ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
ELISA
PRP29 may be used in ELISA applications with AAR29.
Western Blotting
PRP29 may be used under reducing or non-reducing western blotting conditions with AAR29.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Rat Interleukin-10 AAR29 0.1 mg E WB

Product Specific References

References for IL-10

  1. Franz, M. et al. (2015) Targeted delivery of interleukin-10 to chronic cardiac allograft rejection using a human antibody specific to the extra domain A of fibronectin.
    Int J Cardiol. 195: 311-22.