Rat IL-10 is a homodimeric protein, each 18.7 kDa subunit is 160 amino acid residues in length. IL-10 is a potent inhibitor of several cytokines including IFN gamma, IL-2 and TNF.
Interleukin-10 (IL-10) is produced primarily by activated T lymphocytes.
- Target Species
- Product Form
- Purified recombinant protein - lyophilised
- Reconstitute with 10ul water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For extended storage te addtion of 0.1% BSA is recommended,
- Recombinant protein prepared from E.Coli
- Buffer Solution
- Sodium Phosphate
- Preservative Stabilisers
- None present
- Determined by the dose-dependent inhibition of antigen-specific T-cell proliferation is ≤ 10ng/ml
- >98% by SDS PAGE and HPLC analysis
- Approx. Protein Concentrations
- 1.0 mg/ml
- Protein Molecular Weight
- 18.7kD (160 amino acid sequence)
- Endotoxin Level
- < 0.1 ng/ug
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
- 3 months from date of reconstitution.
- Entrez Gene
- GO Terms
negative regulation of cytokine secretion involved in immune response
positive regulation of B cell apoptosis
interleukin-10 receptor binding
response to drug
response to activity
negative regulation of B cell proliferation
response to lipopolysaccharide
negative regulation of interleukin-6 production
receptor biosynthetic process
response to insulin stimulus
response to carbon monoxide
negative regulation of tumor necrosis factor biosynthetic process
negative regulation of apoptosis
positive regulation of gene-specific transcription
negative regulation of nitric oxide biosynthetic process
positive regulation of cytokine secretion
negative regulation of membrane protein ectodomain proteolysis
positive regulation of transcription factor activity
response to glucocorticoid stimulus
regulation of sensory perception of pain
cellular response to estradiol stimulus
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
- PRP29 may be used in ELISA applications with AAR29.
- Western Blotting
- PRP29 may be used under reducing or non-reducing western blotting conditions with AAR29.
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Useful Reagents Available
Product Specific References
References for IL-10
Franz, M. et al. (2015) Targeted delivery of interleukin-10 to chronic cardiac allograft rejection using a human antibody specific to the extra domain A of fibronectin.
Int J Cardiol. 195: 311-22.