IL-10

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Recombinant Rat Interleukin-10

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PRP29 Datasheet	E FN WB SDS	10 µg	Log in		Get Quote

Rat IL-10 is a homodimeric protein, each 18.7 kDa subunit is 160 amino acid residues in length. IL-10 is a potent inhibitor of several cytokines including IFN gamma, IL-2 and TNF.

Interleukin-10 (IL-10) is produced primarily by activated T lymphocytes.

Product Details

Target Species: Rat
Product Form: Purified recombinant protein - lyophilised
Reconstitution: Reconstitute with 10ul water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For extended storage te addtion of 0.1% BSA is recommended,
Preparation: Recombinant protein prepared from E.Coli
Buffer Solution: Sodium Phosphate
Preservative Stabilisers: None present
Carrier Free: Yes
Activity: Determined by the dose-dependent inhibition of antigen-specific T-cell proliferation is ≤ 10ng/ml
Purity: >98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations: 1.0 mg/ml
Protein Molecular Weight: 18.7kD (160 amino acid sequence)
Endotoxin Level: < 0.1 ng/ug

Storage Information

Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at -20^oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 3 months from date of reconstitution

More Information

UniProt: P29456
Entrez Gene: Il10
GO Terms: GO:0002740 negative regulation of cytokine secretion involved in immune response; GO:0002904 positive regulation of B cell apoptosis; GO:0005125 cytokine activity; GO:0005141 interleukin-10 receptor binding; GO:0005615 extracellular space; GO:0006954 inflammatory response; GO:0006955 immune response; GO:0042493 response to drug; GO:0014823 response to activity; GO:0030889 negative regulation of B cell proliferation; GO:0032496 response to lipopolysaccharide; GO:0032715 negative regulation of interleukin-6 production; GO:0032800 receptor biosynthetic process; GO:0032868 response to insulin stimulus; GO:0034465 response to carbon monoxide; GO:0042536 negative regulation of tumor necrosis factor biosynthetic process; GO:0043066 negative regulation of apoptosis; GO:0043193 positive regulation of gene-specific transcription; GO:0045019 negative regulation of nitric oxide biosynthetic process; GO:0050715 positive regulation of cytokine secretion; GO:0051045 negative regulation of membrane protein ectodomain proteolysis; GO:0051091 positive regulation of transcription factor activity; GO:0051384 response to glucocorticoid stimulus; GO:0051930 regulation of sensory perception of pain; GO:0071392 cellular response to estradiol stimulus
Regulatory: For research purposes only

Applications of IL-10

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.2	0.4ng/well
Functional Assays	0.1	10.0ng/ml
Western Blotting	1.5	3.0ng/lane

Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.

ELISA: PRP29 may be used in ELISA applications with AAR29.
Western Blotting: PRP29 may be used under reducing or non-reducing western blotting conditions with AAR29.

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rabbit anti Rat Interleukin-10	AAR29	E WB	0.1 mg	Log in		Get Quote

Product Specific References

References for IL-10

Franz, M. et al. (2015) Targeted delivery of interleukin-10 to chronic cardiac allograft rejection using a human antibody specific to the extra domain A of fibronectin.
Int J Cardiol. 195: 311-22.

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