IL-10
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Rat IL-10 is a homodimeric protein, each 18.7 kDa subunit is 160 amino acid residues in length. IL-10 is a potent inhibitor of several cytokines including IFN gamma, IL-2 and TNF.
Interleukin-10 (IL-10) is produced primarily by activated T lymphocytes.
Interleukin-10 (IL-10) is produced primarily by activated T lymphocytes.
Product Details
- Target Species
- Rat
- Product Form
- Purified recombinant protein - lyophilised
- Reconstitution
- Reconstitute with 10ul water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For extended storage te addtion of 0.1% BSA is recommended, - Preparation
- Recombinant protein prepared from E.Coli
- Buffer Solution
- Sodium Phosphate
- Preservative Stabilisers
- None present
- Activity
- Determined by the dose-dependent inhibition of antigen-specific T-cell proliferation is ≤ 10ng/ml
- Purity
- >98% by SDS PAGE and HPLC analysis
- Approx. Protein Concentrations
- 1.0 mg/ml
- Protein Molecular Weight
- 18.7kD (160 amino acid sequence)
- Endotoxin Level
- <0.1ng/ug
Storage Information
- Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 3 months from date of reconstitution.
More Information
- UniProt
- P29456 Related reagents
- Entrez Gene
- Il10 Related reagents
- GO Terms
- GO:0002740 negative regulation of cytokine secretion involved in immune response
- GO:0002904 positive regulation of B cell apoptosis
- GO:0005125 cytokine activity
- GO:0005141 interleukin-10 receptor binding
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0042493 response to drug
- GO:0014823 response to activity
- GO:0030889 negative regulation of B cell proliferation
- GO:0032496 response to lipopolysaccharide
- GO:0032715 negative regulation of interleukin-6 production
- GO:0032800 receptor biosynthetic process
- GO:0032868 response to insulin stimulus
- GO:0034465 response to carbon monoxide
- GO:0042536 negative regulation of tumor necrosis factor biosynthetic process
- GO:0043066 negative regulation of apoptosis
- GO:0043193 positive regulation of gene-specific transcription
- GO:0045019 negative regulation of nitric oxide biosynthetic process
- GO:0050715 positive regulation of cytokine secretion
- GO:0051045 negative regulation of membrane protein ectodomain proteolysis
- GO:0051091 positive regulation of transcription factor activity
- GO:0051384 response to glucocorticoid stimulus
- GO:0051930 regulation of sensory perception of pain
- GO:0071392 cellular response to estradiol stimulus
- Regulatory
- For research purposes only
Applications of IL-10
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.2 | 0.4ng/well | |
| Functional Assays | 0.1 | 10.0ng/ml | |
| Western Blotting | 1.5 | 3.0ng/lane |
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Rat Interleukin-10 | AAR29 | 0.1 mg | E WB |
Product Specific References
References for IL-10
-
Franz, M. et al. (2015) Targeted delivery of interleukin-10 to chronic cardiac allograft rejection using a human antibody specific to the extra domain A of fibronectin.
Int J Cardiol. 195: 311-22.
