IgG2b | IR863
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Rat IgG2b is derived from a spontaneous immunocytoma originating in a LOU/Wsl rat.
Product Details
- Target Species
- Rat
- Product Form
- Purified myeloma protein - liquid
- Preparation
- Purified protein prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Purity
- Single band by agarose electrophoresis and immunoelectrophoresis
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01835
- P01836
- P20761
- Entrez Gene
- LOC500180
- GO Terms
- GO:0003823 antigen binding
- Regulatory
- For research purposes only
Applications of IgG2b
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.3ug/ml |
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
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Product Specific References
References for IgG2b
-
Edited by Bazin, H. (1990) Rat Hybridomas and Rat Monoclonal Antibodies
CRC Press, Boca Raton, Florida, USA. -
Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
J Immunol Methods. 71 (1): 9-16. -
Argüelles, M.E. et al. (1999) Performance of thiophilic adsorption chromatography in the purification of rat IgG2b monoclonal antibodies from serum- and protein-free culture supernatants.
Biomed Chromatogr. 13 (6): 379-81.
