IgG2b | IR863

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Rat IgG2b Kappa

Product Type: Purified Protein
Clone: IR863
Isotype: IgG2b
Specificity: IgG2b
Region: KAPPA

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References (3)
Applications
Storage Information
Batch/Lot Specific Datasheets

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Rat IgG2b is derived from a spontaneous immunocytoma originating in a LOU/Wsl rat.

Target Species: Rat
Product Form: Purified myeloma protein - liquid
Preparation: Purified protein prepared by affinity chromatography from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
Purity: Single band by agarose electrophoresis and immunoelectrophoresis
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			0.3ug/ml

Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.

References for IgG2b

Edited by Bazin, H. (1990) Rat Hybridomas and Rat Monoclonal Antibodies
CRC Press, Boca Raton, Florida, USA.
Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
J Immunol Methods. 71 (1): 9-16.
Argüelles, M.E. et al. (1999) Performance of thiophilic adsorption chromatography in the purification of rat IgG2b monoclonal antibodies from serum- and protein-free culture supernatants.
Biomed Chromatogr. 13 (6): 379-81.