IgE

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Rat IgE Kappa

Product Type
Purified Protein
Isotype
IgE
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PRP07A E 0.1 mg

Product Details

Target Species
Rat
Product Form
IgE purified (myeloma IR162) - liquid
Preparation
Purified IgE prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
Purity
Single band by agarose eletrophoresis (5) and immunoelectrophoresis (6)
Approx. Protein Concentrations
IgE concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01855 Related reagents
Entrez Gene
Ighe Related reagents
GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of IgE

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.1ug/ml 1.25ug/ml
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.

Product Specific References

References for IgE

  1. Bazin, H. (Ed) (1990) Rat Hybridomas and Rat Monoclonal Antibodies
    CRC Press, Boca Raton, Florida, USA.
  2. Bazin, H. et al. (1973) Brief communication: Transplantable immunoglobulin-secreting tumors in rats. V. Monoclonal immunoglobulins secreted by 250 ileocecal immunocytomas in LOU-Wsl rats.
    J. Natl. Cancer Inst. 51: 1359-1361.
  3. Bazin, H. et al. (1974) Transplantable immunoglobulin-secreting tumours in rats. IV. Sixty-three IgE-secreting immunocytoma tumours.
    Immunology. 26: 713-723.
  4. Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
    J. Immunol. Methods. 71: 9-16.
  5. Bazin, H. J. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
    Immunol. Methods. 66: 261-269.