IgA | IR22

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Rat IgA Kappa

Product Type
Purified Protein
IgA Kappa
Product Code Applications Pack Size List Price Quantity
1 mg loader

Rat IgA is derived from a spontaneous ileocecal immunocytoma in a LOU/Wsl rat (Bazin et al. 1973).

Product Details

Target Species
Product Form
Purified monoclonal IgA - liquid
Purified IgA prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
Single band by agarose electrophoresis (5) and immunoelectrophoresis (6).
Approx. Protein Concentrations
1.0 mg/ml

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

For research purposes only

Applications of IgA

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.15ug/ml 0.6ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate positive/negative controls.

Product Specific References

References for IgA

  1. Edited by Bazin, H. (1990) Rat Hybridomas and Rat Monoclonal Antibodies
    CRC Press. Boca Raton.
  2. Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascites fluid or serum.
    J. Immunol. Methods 71: 9-16.
  3. Bazin, H. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
    J. Immunol. Methods 66: 261-269.
  4. Querinjean, P. et al. (1972) Transplantable immunoglobulin-secreting tumours in rats. Purification and chemical characterization of four kappa chains from LOU-Wsl rats.
    Eur. J. Biochem. 31: 354-359.
  5. Bazin, H. et al. (1973) Brief communication: Transplantable immunoglobulin-secreting tumors in rats. V. Monoclonal immunoglobulins secreted by 250 ileocecal immunocytomas in LOU-Wsl rats
    J. Natl. Cancer Inst. 51: 1359 - 61
  6. Bazin, H. et al. (1974) Allotypes of rat immunoglobulins. I. An allotype at the alpha-chain locus.
    J. Immunology 112: 1035-1041.

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