Collagen I

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Native Rat Collagen I

Product Type
Purified Protein
Product Code Applications Pack Size List Price Quantity
2150-1915
Datasheet
E
MSDS
10 mg loader

Native Rat collagen I is purified rat collagen I derived from tail tendon tissue.

Product Details

Target Species
Rat
Product Form
Purified protein from rat tail tendon - lyophilised
Reconstitution
Reconstitute in ice cold 0.5 M acetic acid, pH 2.5. Solubility - 10 mg/ml.
Preparation
Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I was purified by using differential salt precipitation.
Buffer Solution
Essentially salt free
Preservative Stabilisers
None present.
Purity
SDS PAGE: >90%

Rat collagen type III10%
Rat collagen, other types<1%
Non-collagen proteins<0.5%

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
Do not freeze after reconstitution. Stable for 1-2 months at 4ºC.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P02454
P02466
Entrez Gene
Col1a1
Col1a2
GO Terms
GO:0005615 extracellular space
GO:0009612 response to mechanical stimulus
GO:0031960 response to corticosteroid stimulus
GO:0042542 response to hydrogen peroxide
GO:0043434 response to peptide hormone stimulus
GO:0051591 response to cAMP
GO:0071300 cellular response to retinoic acid
GO:0071363 cellular response to growth factor stimulus
GO:0005201 extracellular matrix structural constituent
Regulatory
For research purposes only

Applications of Collagen I

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Product Specific References

Further Reading

  1. Miller, E.J. & Gay, S. (1982) Collagen: an overview.
    Methods Enzymol. 82 Pt A: 3-32.