CNTF

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Recombinant Rat CNTF

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PRP26 E FN WB 25 µg
Recombinant rat ciliary neurotrophic factor prepared from E. coli.

ciliary neutrophic factor (CNTF) is a neuroregulatory cytokine found primarily in peripheral nerve tissue. CNTF promotes survival and differentiation of neurons and other neuronal cell types.

Product Details

Target Species
Rat
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Centrifuge vial prior to opening. Reconstitute with 25ul of water.

Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. DO NOT VORTEX.
Preparation
Sterile filtered through a 0.2 micron filter, lyophilised from 5mM Sodium Acetate pH 6.5
Preservative Stabilisers
None present
Activity
Determined by it's ability to stimulate the proliferation of human TF-1 cells using a concentration range of 25.0 - 35.0 ng/ml
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
1.0 mg/ml
Protein Molecular Weight
22.7 kDa (199 amino acid sequence)
Endotoxin Level
<0.1ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P20294 Related reagents
Entrez Gene
Cntf Related reagents
GO Terms
GO:0005829 cytosol
GO:0005125 cytokine activity
GO:0005127 ciliary neurotrophic factor receptor binding
GO:0005634 nucleus
GO:0040007 growth
GO:0007259 JAK-STAT cascade
GO:0008083 growth factor activity
GO:0008284 positive regulation of cell proliferation
GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
GO:0046887 positive regulation of hormone secretion
GO:0030307 positive regulation of cell growth
GO:0030424 axon
GO:0032838 cell projection cytoplasm
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0043025 neuronal cell body
GO:0043410 positive regulation of MAPKKK cascade
GO:0043524 negative regulation of neuron apoptosis
GO:0043526 neuroprotection
GO:0048143 astrocyte activation
GO:0048680 positive regulation of axon regeneration
GO:0051291 protein heterooligomerization
GO:0060075 regulation of resting membrane potential
GO:0060081 membrane hyperpolarization
Regulatory
For research purposes only

Applications of CNTF

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
ELISA
PRP26 may be used in ELISA applications with either AAR21 or AAR21B.
Western Blotting
PRP26 may be used in Western blotting applications with either AAR21 or AAR21B.

Product Specific References

References for CNTF

  1. Müller, A. et al. (2007) Astrocyte-derived CNTF switches mature RGCs to a regenerative state following inflammatory stimulation.
    Brain. 130: 3308-20.
  2. Kornfeld, S.F. et al. (2016) Cytoskeletal Linker Protein Dystonin Is Not Critical to Terminal Oligodendrocyte Differentiation or CNS Myelination.
    PLoS One. 11 (2): e0149201.