Choline Acetyltransferase
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Recombinant hepatitis B 320 core antigen 18kDa AYW is a recombinant protein corresponding to rat Choline Acetyltransferase (ChAT), an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
This product may be used as blocking peptide for Goat anti human choline acetyltransferase antibody (product code: 2080-0000) at a suggested concentration range of 2-5 μg/ml. The antigen should be incubated with the antibody for 3 hours at 4°C before being used on tissue sections.
This product may be used as blocking peptide for Goat anti human choline acetyltransferase antibody (product code: 2080-0000) at a suggested concentration range of 2-5 μg/ml. The antigen should be incubated with the antibody for 3 hours at 4°C before being used on tissue sections.
Product Details
- Target Species
- Rat
- Product Form
- Purified recombinant ChAT- liquid
- Preparation
- Purified recombinant rat choline acetyltransferase expressed in Escherichia coli.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Glycerol
- Approx. Protein Concentrations
- Total protein concentration 0.25 mg/ml
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 6 months from date of despatch
More Information
- UniProt
- P32738 Related reagents
- Entrez Gene
- Chat Related reagents
- GO Terms
- GO:0001547 antral ovarian follicle growth
- GO:0001666 response to hypoxia
- GO:0004102 choline O-acetyltransferase activity
- GO:0005737 cytoplasm
- GO:0007584 response to nutrient
- GO:0007613 memory
- GO:0008292 acetylcholine biosynthetic process
- GO:0042493 response to drug
- GO:0019717 synaptosome
- GO:0033265 choline binding
- GO:0045471 response to ethanol
- Regulatory
- For research purposes only
Applications of Choline Acetyltransferase
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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