Choline Acetyltransferase

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Recombinant Rat Choline Acetyltransferase

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2080-0157 C 10 µg
Recombinant hepatitis B 320 core antigen 18kDa AYW is a recombinant protein corresponding to rat Choline Acetyltransferase (ChAT), an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

This product may be used as blocking peptide for Goat anti human choline acetyltransferase antibody (product code: 2080-0000) at a suggested concentration range of 2-5 μg/ml. The antigen should be incubated with the antibody for 3 hours at 4°C before being used on tissue sections.

Product Details

Target Species
Rat
Product Form
Purified recombinant ChAT- liquid
Preparation
Purified recombinant rat choline acetyltransferase expressed in Escherichia coli.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Glycerol
Approx. Protein Concentrations
Total protein concentration 0.25 mg/ml

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
6 months from date of despatch.

More Information

UniProt
P32738 Related reagents
Entrez Gene
Chat Related reagents
GO Terms
GO:0001547 antral ovarian follicle growth
GO:0001666 response to hypoxia
GO:0004102 choline O-acetyltransferase activity
GO:0005737 cytoplasm
GO:0007584 response to nutrient
GO:0007613 memory
GO:0008292 acetylcholine biosynthetic process
GO:0042493 response to drug
GO:0019717 synaptosome
GO:0033265 choline binding
GO:0045471 response to ethanol
Regulatory
For research purposes only

Applications of Choline Acetyltransferase

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.