ACE

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Native Rabbit ACE

Product Type: Purified Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
0100-0313 Datasheet	FN SDS	0.25 Units	Log in		Get Quote

Summary
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Native Rabbit ACE is Rabbit angiotensin converting enzyme purified from lung.
CAS number: 9015-82-1
EC number: 3.4.15.1

Product Details

Target Species: Rabbit
Product Form: Purified protein from rabbit lung - liquid
Preservative Stabilisers: None present
Activity: 4.5 U/mg protein.

One unit will produce 1.0 micromole of hippuric acid from hippuryl-His-Leu per minute in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37ºC.

EDTA can be used as a strong inhibitor of this enzyme. This can be reversed by dialysis and reactivation with metal salts.

The Km for this enzyme is 2.6 mM using hippuryl-L-histidyl-L-leucine as the substrate.
Approx. Protein Concentrations: 10 Units/ml
Protein Molecular Weight: Reported as approximately 129 kDa (sedimentation equilibrium with and without 6 M guanidine hydrochloride) and approximately 140 kDa (SDS gel electrophoresis and by gel filtration).

Storage Information

Storage: Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Guarantee: 12 months from date of despatch

More Information

UniProt: P12822
Entrez Gene: ACE
GO Terms: GO:0004180 carboxypeptidase activity; GO:0008237 metallopeptidase activity; GO:0016021 integral to membrane; GO:0006508 proteolysis
Regulatory: For research purposes only

Applications of ACE

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Functional Assays

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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