ACE

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Native Rabbit ACE

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0100-0313 FN 0.25 Units
Native Rabbit ACE is Rabbit angiotensin converting enzyme purified from lung.
CAS number: 9015-82-1
EC number: 3.4.15.1

Product Details

Target Species
Rabbit
Product Form
Purified protein from rabbit lung - liquid
Preservative Stabilisers
None present
Activity
4.5 U/mg protein.

One unit will produce 1.0 micromole of hippuric acid from hippuryl-His-Leu per minute in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37ºC.

EDTA can be used as a strong inhibitor of this enzyme. This can be reversed by dialysis and reactivation with metal salts.

The Km for this enzyme is 2.6 mM using hippuryl-L-histidyl-L-leucine as the substrate.
Approx. Protein Concentrations
10 Units/ml
Protein Molecular Weight
Reported as approximately 129 kDa (sedimentation equilibrium with and without 6 M guanidine hydrochloride) and approximately 140 kDa (SDS gel electrophoresis and by gel filtration).

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life
18 months from date of despatch

More Information

UniProt
P12822 Related reagents
Entrez Gene
ACE Related reagents
GO Terms
GO:0004180 carboxypeptidase activity
GO:0008237 metallopeptidase activity
GO:0016021 integral to membrane
GO:0006508 proteolysis
Regulatory
For research purposes only

Applications of ACE

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.