Macrophage colony-stimulating factor (CSF1) is the main regulator of macrophage differentiation in all vertebrates (mammals, birds and fish). Mutations in CSF1 and CSF1 receptor (CSF1-R) genes support the view that CSF1-dependent macrophages are essential for normal development and homeostasis. Therefore, numerous potential therapeutic applications of CSF1 have been envisaged (Gow et al. 2010), (Hume et al. 2012). Recombinant CSF1 has been extensively used in studies of macrophage biology, in vitro, to drive the production of macrophages from bone marrow progenitors in culture and to promote the maturation of monocytes (Schroder et al. 2012).
CSF1 has diverged between species, and there is limited cross reactivity between mouse and human. However, the recombinant pig CSF1-Fc protein is active in mouse experiments. The bioactive CSF1 molecule is only 150 amino acids, and when injected, has a short half-life in circulation. The fusion protein of pig CSF1 with the Fc region of pig IgG1a was produced in order to extend the half-life, permitting analysis of the role of the protein in macrophage homeostasis and function. The Fc component had no independent biological activity. And the administration of CSF1-Fc in mice produced a large increase in blood monocytes, and in macrophage numbers throughout the body. The protein was also shown to be safe and efficacious when injected into pigs (Gow et al. 2014).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified recombinant protein - liquid
- Recombinant protein expressed in the egg white of transgenic hens (original stock ISA brown and NovoGen brown), and purified by protein A and size exclusion chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- The observed molecular weight by SDS-PAGE is 98 kDa
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
- 12 months from date of despatch
- For research purposes only
Applications of CSF1-Fc
|Application Name||Verified||Min Dilution||Max Dilution|
Product Specific References
References for CSF1-Fc
Gow, D.J. et al. (2010) CSF-1, IGF-1, and the control of postnatal growth and development.
J Leukoc Biol. 88 (3): 475-81.
Hume, D.A. & Macdonald, K.P. (2012) Therapeutic applications of macrophage colony-stimulating factor-1 (CSF-1) and antagonists of CSF-1 receptor (CSF-1R) signaling.
Blood. 119 (8): 1810-20.
Schroder, K. et al. (2012) Conservation and divergence in Toll-like receptor 4-regulated gene expression in primary human versus mouse macrophages.
Proc Natl Acad Sci U S A. 109 (16): E944-53.
Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
J Immunol. 188 (7): 3382-94.
Gow, D.J. et al. (2014) Characterisation of a novel Fc conjugate of macrophage colony-stimulating factor.
Mol Ther. 22 (9): 1580-92.
Stutchfield, B.M. et al. (2015) CSF1 Restores Innate Immunity After Liver Injury in Mice and Serum Levels Indicate Outcomes of Patients With Acute Liver Failure.
Gastroenterology. 149 (7): 1896-1909.e14.