Stem Cell Factor

100% Secure

Recombinant Mouse Stem Cell Factor

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PMP23 E FN WB 10 µg
Recombinant Mouse stem cell factor (SCF) is a stromal cell derived cytokine that synergizes with other haemapoietic growth factors to stimulate multiple lineages of progenitors e.g. myeloid, erythroid and lymphoid blood cells. The mouse SCF can be used for both mouse and human haemopoietic cells.

Product Details

Target Species
Mouse
Product Form
Purified protein produced in E. coli - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Activity
1 x 105 units/mg
Purity
>95% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
Total protein concentration 0.1mg/ml after reconstitution.
Protein Molecular Weight
18.3kD (164 amino acid residues)
Endotoxin Level
<0.1ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P20826 Related reagents
Entrez Gene
Kitl Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0001755 neural crest cell migration
GO:0002763 positive regulation of myeloid leukocyte differentiation
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005125 cytokine activity
GO:0005173 stem cell factor receptor binding
GO:0005615 extracellular space
GO:0005737 cytoplasm
GO:0005856 cytoskeleton
GO:0007281 germ cell development
GO:0008083 growth factor activity
GO:0033026 negative regulation of mast cell apoptosis
GO:0035234 germ cell programmed cell death
GO:0043406 positive regulation of MAP kinase activity
GO:0045636 positive regulation of melanocyte differentiation
GO:0046579 positive regulation of Ras protein signal transduction
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0070668 positive regulation of mast cell proliferation
Regulatory
For research purposes only

Applications of Stem Cell Factor

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 1 100ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.
ELISA
This product may be used as the standard in an ELISA assay together with either AAM50 or AAM50B
Western Blotting
This product may be used the positive control for western blotting applications with either AAM50 or AAM50B

Product Specific References

References for Stem Cell Factor

  1. Gilicze, A. et al. (2007) HtrA1 is a novel mast cell serine protease of mice and men.
    Mol Immunol. 44: 2961-8.
  2. Wiener, Z. et al. (2003) Mouse embryonic stem cells express histidine decarboxylase and histamine H1 receptors.
    Inflamm Res. 52 Suppl 1:S53-4.
  3. Casazza, A. et al. (2011) Systemic and targeted delivery of semaphorin 3A inhibits tumor angiogenesis and progression in mouse tumor models.
    Arterioscler Thromb Vasc Biol. 31: 741-9.
  4. Jouneau, A. et al. (2000) Plasticity of cadherin-catenin expression in the melanocyte lineage.
    Pigment Cell Res. 13: 260-72.