SDF-1 Alpha

100% Secure

Recombinant Mouse SDF-1 Alpha

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PMP77 FN 10 µg
SDF-1 alpha, otherwise known as CXCL12a, is a stromal cell derived CXC chemokine which, together with SDF-1 beta, arises from alternative splicing of the SDF-1 gene, and acts as a chemoattractant for T and B lymphocytes, monocytes and migratory neurons.

SDF-1 binds with high-affinity to the G protein–coupled receptor CXCR4 (fusin), and acts as an inhibitor of the CXCR4-mediated entry of HIV-1 virus into target T-cells.

Product Details

Target Species
Mouse
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 100ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant SDF-1 alpha expressed in E.coli
Preservative Stabilisers
None present
Activity
Determined by its ability to chemoattract human peripheral blood monocytes, using a concentration of 50-100 ng/ml.
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1mg/ml
Molecular Weight
7.9kD (68 amino acid residue homodimer)
Endotoxin Level
<1EU/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P40224 Related reagents
Entrez Gene
Cxcl12 Related reagents
GO Terms
GO:0001569 patterning of blood vessels
GO:0001667 ameboidal cell migration
GO:0005615 extracellular space
GO:0006955 immune response
GO:0007281 germ cell development
GO:0008009 chemokine activity
GO:0008045 motor axon guidance
GO:0008083 growth factor activity
GO:0008354 germ cell migration
GO:0009897 external side of plasma membrane
GO:0030335 positive regulation of cell migration
GO:0042098 T cell proliferation
GO:0050930 induction of positive chemotaxis
Regulatory
For research purposes only

Applications of SDF-1 Alpha

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.