MIP-1 Alpha

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Recombinant Mouse MIP-1 Alpha

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PMP32 E FN WB 10 µg
Murine macrophage inflammatory protein-1 alpha (MIP-1 alpha), also known as CCL3, is a member of the chemokine family (C-C) and plays an important role in the inflammatory response of blood monocytes and tissue macrophages.

Product Details

Target Species
Mouse
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.1% bovine serum albumin is recommended.
Preparation
Recombinant protein prepared from E.Coli
Preservative Stabilisers
None present
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Protein Molecular Weight
7.8kD (69 amino acid sequence)
Endotoxin Level
<0.1 ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P10855 Related reagents
Entrez Gene
Ccl3 Related reagents
GO Terms
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0008009 chemokine activity
Regulatory
For research purposes only

Applications of MIP-1 Alpha

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 10 100ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
ELISA
PMP32 may be used in ELISA applications with AAM46B.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Mouse MIP-1 Alpha AAM46 0.1 mg E WB