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IL-4

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Recombinant Mouse Interleukin-4 thumbnail image 1
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Published customer image:
Murine macrophages cultured in the presence of mouse interferon-γ (PMP43A ) or mouse interleukin-4 (PMP81) to generate an M1 and M2 phenotype respectively. cells were sublequently exposed to bacteria as indicated to visualise phagocytosis.
Image caption:
3D-SIM images of P. gingivalis W50 phagocytosed by naive, M1 and M2 macrophages.
(A,B,C) Maximum intensity projection and (D,E,F) the corresponding slice view 3D-SIM images of P. gingivalis W50 phagocytosed by naive, M1 and M2 macrophages (60:1 BMR, 1 hour exposure time), respectively, on the Deltavision OMX Structured Illumination Microscope V4 Blaze (Applied Precision, WA, USA). P. gingivalis W50 were labelled with AF488 (green) while the nuclei and actin filaments of the macrophages were stained with DAPI (blue) and Phalloidin-TRITC (red) respectively. The slice images were obtained from a section with defined size (between 10–50 z-stack) to confirm phagocytosis of P. gingivalis W50 by naive, M1 and M2 macrophages.

From: Lam RS, O'Brien-Simpson NM, Holden JA, Lenzo JC, Fong SB, Reynolds EC (2016)
Unprimed, M1 and M2 Macrophages Differentially Interact with Porphyromonas gingivalis.
PLoS ONE 11(7): e0158629.

Recombinant Mouse Interleukin-4

Product Type
Recombinant Protein
Specificity
IL-4

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Product Code Applications Pack Size List Price Your Price Qty
PMP81
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SDS Safety Datasheet SDS
E FN WB 20 µg loader
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Recombinant Murine interleukin-4 is an E.coli derived preparation of Interleukin-4 (IL-4), also known as B-cell stimulatory factor 1. Interleukin-4 is a pleiotropic cytokine which interacts with multiple cell lineages, including T cells, fibroblasts and haematopoietic cells, and is one of the most important factors involved in B cell IgG1 and IgE class switching. IL-4 acts as a regulator of low affinity Fc receptor for IgE (CD23) expression on both lymphocytes and monocytes.

Target Species
Mouse
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 200 ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant IL-4 expressed in E.coli
Source
E.coli
Preservative Stabilisers
None present
Carrier Free
Yes
Activity
The ED50 was determined by the dose-dependent proliferation of murine HT-2 cells is < 2.0 ng/ml, corresponding to a specific activity of > 5 x 105 units/mg.
Purity
>98% by SDS PAGE and HPLC
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Protein Molecular Weight
13.5 kDa (120 amino acid residues)
Endotoxin Level
< 1.0 EU/ug
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
PMP81 may be used as a standard in an ELISA application with AAM36.
Western Blotting
PMP81 may be used as a positive control for western blotting applications with AAM36.

References for IL-4

  1. Thomas, M.J. et al. (2002) CD8 T cells inhibit IgE via dendritic cell IL-12 induction that promotes Th1 T cell counter-regulation.
    J Immunol. 168 (1): 216-23.
  2. Bénéteau, M. et al. (2012) Combination of glycolysis inhibition with chemotherapy results in an antitumor immune response.
    Proc Natl Acad Sci U S A. 109: 20071-6.
  3. Lam, R.S. et al. (2016) Unprimed, M1 and M2 Macrophages Differentially Interact with Porphyromonas gingivalis.
    PLoS One. 11 (7): e0158629.

UniProt
P07750
Entrez Gene
Il4
GO Terms
GO:0005125 cytokine activity
GO:0005136 interleukin-4 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0008203 cholesterol metabolic process
GO:0030890 positive regulation of B cell proliferation
GO:0042104 positive regulation of activated T cell proliferation
GO:0042113 B cell activation
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0043066 negative regulation of apoptosis
GO:0043193 positive regulation of gene-specific transcription
GO:0045348 positive regulation of MHC class II biosynthetic process
GO:0045671 negative regulation of osteoclast differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048295 positive regulation of isotype switching to IgE isotypes
GO:0048304 positive regulation of isotype switching to IgG isotypes
GO:0051091 positive regulation of transcription factor activity

PMP81

151336 166834 167154

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