Recombinant Murine interleukin-4 is an E.coli derived preparation of Interleukin-4 (IL-4), also known as B-cell stimulatory factor 1. Interleukin-4 is a pleiotropic cytokine which interacts with multiple cell lineages, including T cells, fibroblasts and haematopoietic cells, and is one of the most important factors involved in B cell IgG1 and IgE class switching. IL-4 acts as a regulator of low affinity Fc receptor for IgE (CD23) expression on both lymphocytes and monocytes.
- Target Species
- Product Form
- Purified recombinant protein - lyophilised
- Reconstitute with 200ul distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Purified recombinant IL-4 expressed in E.coli
- Preservative Stabilisers
- None present
- The ED50 was determined by the dose-dependent proliferation of murine HT-2 cells is < 2.0 ng/ml, corresponding to a specific activity of > 5 x 105 units/mg.
- >98% by SDS PAGE and HPLC analysis
- Approx. Protein Concentrations
- 0.1mg/ml after reconstitution
- Molecular Weight
- 13.5kD (120 amino acid residues)
- Endotoxin Level
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 3 months from date of reconstitution.
- P07750 Related reagents
- Entrez Gene
- Il4 Related reagents
- GO Terms
- GO:0005125 cytokine activity
- GO:0005136 interleukin-4 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0008203 cholesterol metabolic process
- GO:0030890 positive regulation of B cell proliferation
- GO:0042104 positive regulation of activated T cell proliferation
- GO:0042113 B cell activation
- GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
- GO:0043066 negative regulation of apoptosis
- GO:0043193 positive regulation of gene-specific transcription
- GO:0045348 positive regulation of MHC class II biosynthetic process
- GO:0045671 negative regulation of osteoclast differentiation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048295 positive regulation of isotype switching to IgE isotypes
- GO:0048304 positive regulation of isotype switching to IgG isotypes
- GO:0051091 positive regulation of transcription factor activity
- For research purposes only
Applications of IL-4
|Application Name||Verified||Min Dilution||Max Dilution|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for IL-4
Thomas, M.J. et al. (2002) CD8 T cells inhibit IgE via dendritic cell IL-12 induction that promotes Th1 T cell counter-regulation.
J Immunol. 168 (1): 216-23.
Bénéteau, M. et al. (2012) Combination of glycolysis inhibition with chemotherapy results in an antitumor immune response.
Proc Natl Acad Sci U S A. 109: 20071-6.
Lam, R.S. et al. (2016) Unprimed, M1 and M2 Macrophages Differentially Interact with Porphyromonas gingivalis.
PLoS One. 11 (7): e0158629.