IL-12

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Recombinant Mouse Interleukin-12

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PMP40 E FN WB 10 µg
Recombinant mouse IL-12 is a 70.5kDa heterodimeric glycoprotein consisting of disulfide-linked p35 and p40 subunits. IL-12 is a potent regulator of cell-mediated immune responses. It induces production of interferon gamma in natural killer and T-cells.

Product Details

Target Species
Mouse
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.1ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in CHO cells.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Activity
1 x 107 units/mg
Purity
>98% by SDS PAGE + HPLC analysis
Approx. Protein Concentrations
0.1 mg/ml after reconstitution.
Protein Molecular Weight
75kD (35kDa and 40kDa subunits) - 506 amino acid residues
Endotoxin Level
<0.1 ng/ug

Storage Information

Storage
Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

Please see label for expiry date.

More Information

UniProt
P43431 Related reagents
P43432 Related reagents
Entrez Gene
Il12a Related reagents
Il12b Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005143 interleukin-12 receptor binding
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0032729 positive regulation of interferon-gamma production
GO:0042102 positive regulation of T cell proliferation
GO:0042163 interleukin-12 beta subunit binding
GO:0042832 defense response to protozoan
GO:0043514 interleukin-12 complex
GO:0001916 positive regulation of T cell mediated cytotoxicity
GO:0002230 positive regulation of defense response to virus by host
GO:0002827 positive regulation of T-helper 1 type immune response
GO:0002862 negative regulation of inflammatory response to antigenic stimulus
GO:0016020 membrane
GO:0004896 cytokine receptor activity
GO:0044130 negative regulation of growth of symbiont in host
GO:0042093 T-helper cell differentiation
GO:0042104 positive regulation of activated T cell proliferation
GO:0042164 interleukin-12 alpha subunit binding
GO:0042803 protein homodimerization activity
Regulatory
For research purposes only

Applications of IL-12

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 1.0ng/ml 10.0ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.
ELISA
Recombinant mouse IL-12 may be used as the standard in ELISA application with either a purified mouse IL-12 antibody (AAM33) or a biotinylated mouse IL-12 antibody (AAM33B).
Western Blotting
Recombinant mouse IL-12 may be used as a positive control for Western Blotting applications with either a purified mouse IL-12 antibody (AAM33) or a biotinylated mouse IL-12 antibody (AAM33B).