IgG2b | MADNP-3

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Mouse IgG2b Kappa

Product Type
Purified Protein
Clone
MADNP-3
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
PMP50
Datasheet
E
SDS
1 mg loader

Product Details

Target Species
Mouse
Product Form
Purified monoclonal IgG2b - liquid
Preparation
Purified IgG2b prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
Purity
Single band by agarose electrophoresis and immunoelectrophoresis.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P01867
P01837
Entrez Gene
Igk-C
Ighg2b
GO Terms
GO:0003823 antigen binding
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
Regulatory
For research purposes only

Applications of IgG2b

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Product Specific References

References for IgG2b

  1. Gustafsson, A. et al. (2011) Pichia pastoris-produced mucin-type fusion proteins with multivalent O-glycan substitution as targeting molecules for mannose-specific receptors of the immune system.
    Glycobiology. 21: 1071-86.
  2. Flowers, S.A. et al. (2020) Decrease of core-2 O-glycans on synovial lubricin in osteoarthritis reduces galectin-3 mediated crosslinking
    J Biol Chem : jbc.RA120.012882. [Epub ahead of print]

Fluorescent Spectraviewer

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How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra