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EGF

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Recombinant Mouse EGF thumbnail image 1
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Western Blot analysis 1 μg recombinant mouse EGF (PMP89) was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody (AAM52) was used at 0.1 μg/ml (lane 2) and 0.2 μg/ml (lane 3). HRP conjugated Donkey anti Sheep/Goat IgG antibody (STAR88P) was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc Touch Imaging System.
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Recombinant Mouse EGF

Product Type
Recombinant Protein
Specificity
EGF

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PMP89
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Epidermal Growth Factor (EGF) is a type I transmembrane protein expressed by a variety of cells, which acts as a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, and is a key factor in cell survival.
EGF is a magnesiotropic hormone involved in the regulation of renal magnesium reabsorption via activation of the magnesium permeable channel TRPM6 (Muallem and Moe 2007).
The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the EGF receptor (EGFR), which results in the triggering of several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways.

Target Species
Mouse
Product Form
Purified recombinant protein – lyophilized
Reconstitution
Reconstitute to 1.0 mg/ml by adding 1.0 ml H2O. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Do not vortex.
Preparation
Purified recombinant EGF expressed in E.coli
Preservative Stabilisers
None present
Activity
The ED50 as determined by a cell proliferation assay using BALB/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
1.0 mg/ml after reconstitution
Protein Molecular Weight
6 kDa
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for EGF

  1. Kite, J. et al. (2021) Cell-to-cell transmission of HSV1 in human keratinocytes in the absence of the major entry receptor, nectin1.
    PLoS Pathog. 17 (9): e1009631.

Further Reading

  1. Muallem, S. & Moe, O.W. (2007) When EGF is offside, magnesium is wasted.
    J Clin Invest. 117 (8): 2086-9.

UniProt
P01132
Entrez Gene
Egf
GO Terms
GO:0005886 plasma membrane
GO:0000186 activation of MAPKK activity
GO:0005509 calcium ion binding
GO:0016021 integral to membrane
GO:0005154 epidermal growth factor receptor binding
GO:0005615 extracellular space
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0008083 growth factor activity
GO:0018108 peptidyl-tyrosine phosphorylation
GO:0021940 positive regulation of cerebellar granule cell precursor proliferation
GO:0048754 branching morphogenesis of a tube
GO:0050730 regulation of peptidyl-tyrosine phosphorylation
GO:0060749 mammary gland alveolus development
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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