CXCL1

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Recombinant Mouse CXCL1

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PMP66 Datasheet	E FN WB SDS	20 µg	Log in		Get Quote

CXCL1, also known as GRO1, is a 7.8 kDa chemokine which is involved in neutrophil chemotaxis and activation and is expressed in a range of cells including monocytes, macrophages and vascular endothelial cells.

Product Details

Target Species: Mouse
Product Form: Purified Recombinant Protein - lyophilized
Reconstitution: Reconstitute with 20 ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Recombinant protein expressed in E.coli
Preservative Stabilisers: None present
Carrier Free: Yes
Purity: >98% by SDS page and HPLC analysis
Approx. Protein Concentrations: 1.0 mg/ml after reconstitution
Protein Molecular Weight: 7.8 kDa (72 amino acid sequence)
Endotoxin Level: < 0.1 ng/ug

Storage Information

Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at -20^oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 3 months from date of reconstitution

More Information

UniProt: P12850
Entrez Gene: Cxcl1
GO Terms: GO:0005615 extracellular space; GO:0006954 inflammatory response; GO:0006955 immune response; GO:0008009 chemokine activity; GO:0008083 growth factor activity
Regulatory: For research purposes only

Applications of CXCL1

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.2	0.4ng/well
Functional Assays	0.01	0.10ug/ml
Western Blotting	1.5	3.0ng/lane

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: PMP66 may be used in ELISA applications with either AAM40 or AAM40B.
Western Blotting: PMP66 may be used under reducing or non-reducing western blotting conditions with either AAM40 or AAM40B.

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rabbit anti Mouse CXCL1	AAM40	E WB	0.1 mg	Log in		Get Quote
Rabbit anti Mouse CXCL1:Biotin	AAM40B	E WB	50 µg	Log in		Get Quote

Product Specific References

References for CXCL1

Van de Walle, G.R. et al. (2007) Herpesvirus chemokine-binding glycoprotein G (gG) efficiently inhibits neutrophil chemotaxis in vitro and in vivo.
J Immunol. 179: 4161-9.
Finsterbusch, M. et al. (2014) Neutrophils recruited by chemoattractants in vivo induce microvascular plasma protein leakage through secretion of TNF.
J Exp Med. 211: 1307-14.

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