CXCL1

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Recombinant Mouse CXCL1

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PMP66 E FN WB 20 µg
CXCL1, also known as GRO1, is a 7.8kD chemokine which is involved in neutrophil chemotaxis and activation and is expressed in a range of cells including monocytes, macrophages and vascular endothelial cells.

Product Details

Target Species
Mouse
Product Form
Purified Recombinant Protein - lyophilised
Reconstitution
Reconstitute with 20ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in E.coli
Preservative Stabilisers
None present
Purity
>98% by SDS page and HPLC analysis
Approx. Protein Concentrations
1.0mg/ml after reconstitution
Protein Molecular Weight
7.8kD (72 amino acid sequence)
Endotoxin Level
<0.1ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P12850 Related reagents
Entrez Gene
Cxcl1 Related reagents
GO Terms
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0008009 chemokine activity
GO:0008083 growth factor activity
Regulatory
For research purposes only

Applications of CXCL1

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 0.01 0.10ug/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
PMP66 may be used in ELISA applications with either AAM40 or AAM40B.
Western Blotting
PMP66 may be used under reducing or non-reducing western blotting conditions with either AAM40 or AAM40B.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Mouse CXCL1 AAM40 0.1 mg E WB
Rabbit anti Mouse CXCL1:Biotin AAM40B 50 µg E WB

Product Specific References

References for CXCL1

  1. Van de Walle, G.R. et al. (2007) Herpesvirus chemokine-binding glycoprotein G (gG) efficiently inhibits neutrophil chemotaxis in vitro and in vivo.
    J Immunol. 179: 4161-9.
  2. Finsterbusch, M. et al. (2014) Neutrophils recruited by chemoattractants in vivo induce microvascular plasma protein leakage through secretion of TNF.
    J Exp Med. 211: 1307-14.