CXCL1
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CXCL1, also known as GRO1, is a 7.8kD chemokine which is involved in neutrophil chemotaxis and activation and is expressed in a range of cells including monocytes, macrophages and vascular endothelial cells.
Product Details
- Target Species
- Mouse
- Product Form
- Purified Recombinant Protein - lyophilised
- Reconstitution
- Reconstitute with 20ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in E.coli
- Preservative Stabilisers
- None present
- Purity
- >98% by SDS page and HPLC analysis
- Approx. Protein Concentrations
- 1.0mg/ml after reconstitution
- Protein Molecular Weight
- 7.8kD (72 amino acid sequence)
- Endotoxin Level
- <0.1ng/ug
Storage Information
- Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 3 months from date of reconstitution.
More Information
- UniProt
- P12850 Related reagents
- Entrez Gene
- Cxcl1 Related reagents
- GO Terms
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0008009 chemokine activity
- GO:0008083 growth factor activity
- Regulatory
- For research purposes only
Applications of CXCL1
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.2 | 0.4ng/well | |
| Functional Assays | 0.01 | 0.10ug/ml | |
| Western Blotting | 1.5 | 3.0ng/lane |
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Mouse CXCL1 | AAM40 | 0.1 mg | E WB | ||
| Rabbit anti Mouse CXCL1:Biotin | AAM40B | 50 µg | E WB |
Product Specific References
References for CXCL1
-
Van de Walle, G.R. et al. (2007) Herpesvirus chemokine-binding glycoprotein G (gG) efficiently inhibits neutrophil
chemotaxis in vitro and in vivo.
J Immunol. 179: 4161-9. -
Finsterbusch, M. et al. (2014) Neutrophils recruited by chemoattractants in vivo induce microvascular plasma protein leakage through secretion of TNF.
J Exp Med. 211: 1307-14.
