Wnt-1, which signals through binding to the Frizzled family of transmembrane receptors, was originally identified as a proto-oncogene implicated in carcinomas induced by the mouse mammary tumour virus (MMTV). Wnt-1 belongs to a family of Wnt genes involved in the dorso-ventricular development of the mesoderm during embryogenesis, with a restricted temporal and spatial pattern of expression.
- Target Species
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitute with 100 ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Purified recombinant Wnt-1 expressed in E.coli
- Preservative Stabilisers
- None present
- The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.
- >98% by SDS PAGE and HPLC
- Approx. Protein Concentrations
- 0.1 mg/ml
- Protein Molecular Weight
- 38.4 kDa (343 amino acid residues)
- Endotoxin Level
- < 1.0 EU/ug
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 3 months from date of reconstitution
- Entrez Gene
- GO Terms
- GO:0005886 plasma membrane
- GO:0004871 signal transducer activity
- GO:0007223 Wnt receptor signaling pathway, calcium modulating pathway
- GO:0005109 frizzled binding
- GO:0005110 frizzled-2 binding
- GO:0005578 proteinaceous extracellular matrix
- GO:0005615 extracellular space
- GO:0005769 early endosome
- GO:0005770 late endosome
- GO:0009611 response to wounding
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010592 positive regulation of lamellipodium assembly
- GO:0010812 negative regulation of cell-substrate adhesion
- GO:0010843 promoter binding
- GO:0016563 transcription activator activity
- GO:0021536 diencephalon development
- GO:0021551 central nervous system morphogenesis
- GO:0021588 cerebellum formation
- GO:0021592 fourth ventricle development
- GO:0021797 forebrain anterior/posterior pattern formation
- GO:0022004 midbrain-hindbrain boundary maturation during brain development
- GO:0022408 negative regulation of cell-cell adhesion
- GO:0030901 midbrain development
- GO:0033077 T cell differentiation in thymus
- GO:0035284 brain segmentation
- GO:0042472 inner ear morphogenesis
- GO:0042770 signal transduction in response to DNA damage
- GO:0043568 positive regulation of insulin-like growth factor receptor signaling pathway
- GO:0044336 canonical Wnt receptor signaling pathway involved in negative regulation of apoptosis
- GO:0045121 membrane raft
- GO:0045599 negative regulation of fat cell differentiation
- GO:0045747 positive regulation of Notch signaling pathway
- GO:0048146 positive regulation of fibroblast proliferation
- GO:0048332 mesoderm morphogenesis
- GO:0048664 neuron fate determination
- GO:0061054 dermatome development
- GO:0070365 hepatocyte differentiation
- GO:0071425 hemopoietic stem cell proliferation
- GO:2000002 negative regulation of DNA damage checkpoint
- For research purposes only
Applications of WNT-1
|Application Name||Verified||Min Dilution||Max Dilution|
Product Specific References
References for WNT-1
Gherghe, C.M. et al. (2011) Wnt1 is a proangiogenic molecule, enhances human endothelial progenitor function, and increases blood flow to ischemic limbs in a HGF-dependent manner.
FASEB J. 25: 1836-43.
Maguschak, K.A. & Ressler, K.J. (2011) Wnt signaling in amygdala-dependent learning and memory.
J Neurosci. 31 (37): 13057-67.