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WNT-1

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Recombinant Human WNT-1

Product Type
Recombinant Protein
Specificity
WNT-1

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Product Code Applications Pack Size List Price Your Price Qty
PHP183
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
FN 10 µg
List Price Your Price

Wnt-1, formerly known as int-1, is a non-glycosylated secreted protein associated with the extracellular matrix, involved in normal embryonic development and particularly linked with breast cancer.

Wnt-1, which signals through binding to the Frizzled family of transmembrane receptors, was originally identified as a proto-oncogene implicated in carcinomas induced by the mouse mammary tumour virus (MMTV). Wnt-1 belongs to a family of Wnt genes involved in the dorso-ventricular development of the mesoderm during embryogenesis, with a restricted temporal and spatial pattern of expression.

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 100 ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant Wnt-1 expressed in E.coli
Source
E.coli
Preservative Stabilisers
None present
Carrier Free
Yes
Activity
The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.
Purity
>98% by SDS PAGE and HPLC
Approx. Protein Concentrations
0.1 mg/ml
Protein Molecular Weight
38.4 kDa (343 amino acid residues)
Endotoxin Level
< 1.0 EU/ug
Regulatory
For research purposes only
Guarantee
3 months from date of reconstitution

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for WNT-1

  1. Gherghe, C.M. et al. (2011) Wnt1 is a proangiogenic molecule, enhances human endothelial progenitor function, and increases blood flow to ischemic limbs in a HGF-dependent manner.
    FASEB J. 25: 1836-43.
  2. Maguschak, K.A. & Ressler, K.J. (2011) Wnt signaling in amygdala-dependent learning and memory.
    J Neurosci. 31 (37): 13057-67.

Synonyms
WNT-1 Proto-Oncogene Protein
UniProt
P04628
Entrez Gene
WNT1
GO Terms
GO:0005886 plasma membrane
GO:0004871 signal transducer activity
GO:0007223 Wnt receptor signaling pathway, calcium modulating pathway
GO:0005109 frizzled binding
GO:0005110 frizzled-2 binding
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0005769 early endosome
GO:0005770 late endosome
GO:0009611 response to wounding
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010592 positive regulation of lamellipodium assembly
GO:0010812 negative regulation of cell-substrate adhesion
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0021536 diencephalon development
GO:0021551 central nervous system morphogenesis
GO:0021588 cerebellum formation
GO:0021592 fourth ventricle development
GO:0021797 forebrain anterior/posterior pattern formation
GO:0022004 midbrain-hindbrain boundary maturation during brain development
GO:0022408 negative regulation of cell-cell adhesion
GO:0030901 midbrain development
GO:0033077 T cell differentiation in thymus
GO:0035284 brain segmentation
GO:0042472 inner ear morphogenesis
GO:0042770 signal transduction in response to DNA damage
GO:0043568 positive regulation of insulin-like growth factor receptor signaling pathway
GO:0044336 canonical Wnt receptor signaling pathway involved in negative regulation of apoptosis
GO:0045121 membrane raft
GO:0045599 negative regulation of fat cell differentiation
GO:0045747 positive regulation of Notch signaling pathway
GO:0048146 positive regulation of fibroblast proliferation
GO:0048332 mesoderm morphogenesis
GO:0048664 neuron fate determination
GO:0061054 dermatome development
GO:0070365 hepatocyte differentiation
GO:0071425 hemopoietic stem cell proliferation
GO:2000002 negative regulation of DNA damage checkpoint
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