VEGF is a homodimeric protein produced by various vascularized tissues. Activities include stimulation of endothelial cell growth, angiogenesis and capillary permeability.
- Target Species
- Product Form
- Purified recombinant protein expressed in E. coli - lyophilised
- Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Preservative Stabilisers
- None present
- >98% by SDS PAGE and HPLC analysis
- Approx. Protein Concentrations
- 0.1 mg/ml
- Protein Molecular Weight
- 38kD (2 x 165 Amino acid sequence)
- Endotoxin Level
- <0.1 ng/ug
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 12 months from date of reconstitution.
- P15692 Related reagents
- Entrez Gene
- VEGFA Related reagents
- For research purposes only
Applications of VEGF
|Application Name||Verified||Min Dilution||Max Dilution|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Goat anti Human VEGF||AHP831||0.1 mg||E FN P* WB|
|Goat anti Human VEGF:Biotin||AHP831B||50 µg||E WB|
Product Specific References
References for VEGF
Easton, C.D. et al. (2014) Application of layer-by-layer coatings to tissue scaffolds – development of an angiogenic biomaterial.
Journal of Materials Chemistry B. 2, 5558-68