PDGF

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Recombinant Human PDGF AA Homodimer

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP035 FN 10 µg
Recombinant Human Platelet Derived Growth Factor (PDGF) is a potent mitogen, acting on cell types such as fibroblasts and smooth muscle cells.PDGF is a dimeric protein being composed of A and B chains. The dimer may be formed from AA, BB or AB chain combinations.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 10ul of 10mM acetic acid. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein prepared from E.Coli
Preservative Stabilisers
None present
Purity
>98% by SDS PAGE and N-terminal sequence analysis.
Approx. Protein Concentrations
1.0mg/ml
Protein Molecular Weight
26.5kDa (250 amino acid sequence)
Endotoxin Level
<0.1ng/mg

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P04085 Related reagents
Entrez Gene
PDGFA Related reagents
GO Terms
GO:0000139 Golgi membrane
GO:0002576 platelet degranulation
GO:0005161 platelet-derived growth factor receptor binding
GO:0005518 collagen binding
GO:0005615 extracellular space
GO:0005788 endoplasmic reticulum lumen
GO:0009790 embryo development
GO:0008083 growth factor activity
GO:0009986 cell surface
GO:0010512 negative regulation of phosphatidylinositol biosynthetic process
GO:0010544 negative regulation of platelet activation
GO:0014910 regulation of smooth muscle cell migration
GO:0030168 platelet activation
GO:0030335 positive regulation of cell migration
GO:0031093 platelet alpha granule lumen
GO:0032956 regulation of actin cytoskeleton organization
GO:0042803 protein homodimerization activity
GO:0043498 cell surface binding
GO:0045740 positive regulation of DNA replication
GO:0046982 protein heterodimerization activity
GO:0048008 platelet-derived growth factor receptor signaling pathway
GO:0048146 positive regulation of fibroblast proliferation
GO:0048407 platelet-derived growth factor binding
GO:0050919 negative chemotaxis
GO:0051781 positive regulation of cell division
Regulatory
For research purposes only

Applications of PDGF

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays 0.2ng/ml 10ng/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.