PDGF

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Recombinant Human PDGF AA Homodimer

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PHP035 Datasheet	FN SDS	10 µg	Log in		Get Quote

Summary
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Recombinant Human Platelet Derived Growth Factor (PDGF) is a potent mitogen, acting on cell types such as fibroblasts and smooth muscle cells.PDGF is a dimeric protein being composed of A and B chains. The dimer may be formed from AA, BB or AB chain combinations.

Product Details

Target Species: Human
Product Form: Purified recombinant protein - lyophilised
Reconstitution: Reconstitute with 10ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Recombinant protein prepared from E.Coli
Buffer Solution: 10mM Acetic Acid
Preservative Stabilisers: None present
Carrier Free: Yes
Purity: >98% by SDS PAGE and N-terminal sequence analysis.
Approx. Protein Concentrations: 1.0 mg/ml after reconstitution
Protein Molecular Weight: 26.5kDa (250 amino acid sequence)
Endotoxin Level: < 0.1 ng/mg

Storage Information

Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at -20^oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 3 months from date of reconstitution

More Information

UniProt: P04085
Entrez Gene: PDGFA
GO Terms: GO:0000139 Golgi membrane; GO:0002576 platelet degranulation; GO:0005161 platelet-derived growth factor receptor binding; GO:0005518 collagen binding; GO:0005615 extracellular space; GO:0005788 endoplasmic reticulum lumen; GO:0009790 embryo development; GO:0008083 growth factor activity; GO:0009986 cell surface; GO:0010512 negative regulation of phosphatidylinositol biosynthetic process; GO:0010544 negative regulation of platelet activation; GO:0014910 regulation of smooth muscle cell migration; GO:0030168 platelet activation; GO:0030335 positive regulation of cell migration; GO:0031093 platelet alpha granule lumen; GO:0032956 regulation of actin cytoskeleton organization; GO:0042803 protein homodimerization activity; GO:0043498 cell surface binding; GO:0045740 positive regulation of DNA replication; GO:0046982 protein heterodimerization activity; GO:0048008 platelet-derived growth factor receptor signaling pathway; GO:0048146 positive regulation of fibroblast proliferation; GO:0048407 platelet-derived growth factor binding; GO:0050919 negative chemotaxis; GO:0051781 positive regulation of cell division
Regulatory: For research purposes only

Applications of PDGF

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Functional Assays		0.2ng/ml	10ng/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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