Neuron Specific Enolase

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Native Human Neuron Specific Enolase

Product Type: Purified Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
6720-2004 Datasheet	E SDS	50 µg	Log in		Get Quote

Purified neuron specific enolase (NSE) has been prepared from human brain tissue by ion exchange chroatography and gel filtration. NSE, also known as gama enolase, 2-phospho-D-glycerate hydro-lyase, enolase-2 or Neural enolase, is a glycolytic enzyme expressed in neuronal tissue and released in response to neural tissue damage. An increase in serum levels of NSE has been found in patients with tumors such as neuroblastoma (Odelstad et al. 1981, Zeltzer et al. 1983).

Product Details

Target Species: Human
Product Form: Purified enzyme from human brain - liquid
Preparation: Ion exchange chromatography and gel filtration.
Buffer Solution: 0.1M TRIS/HCl
0.005M Magnesium Sulfate
0.25M Potassium Chloride
Preservative Stabilisers: None present
Activity: 58 µmol/min mg at 25ºC determined in 45 mM Imidasole, pH 6.8, 45 mM KCl, 1.33 mM Mg₂SO₄, 1 mM 2-PG.
Purity: SDS PAGE: >95%
Approx. Protein Concentrations: Total protein concentration 0.87 mg/ml

Storage Information

Storage: Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: P09104
Entrez Gene: ENO2
GO Terms: GO:0000015 phosphopyruvate hydratase complex; GO:0005886 plasma membrane; GO:0000287 magnesium ion binding; GO:0004634 phosphopyruvate hydratase activity; GO:0006094 gluconeogenesis; GO:0006096 glycolysis
Regulatory: For research purposes only

Applications of Neuron Specific Enolase

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Product Specific References

References for Neuron Specific Enolase

Hullin, D.A. et al. (1980) Purification, radioimmunoassay, and distribution of human brain 14-3-2 protein (nervous-system specific enolase) in human tissues.
Biochim Biophys Acta. 628 (1): 98-108.
Cheng, S. et al. (2015) Label-free detection of tumor markers using field effect transistor (FET)-based biosensors for lung cancer diagnosis
Sensors and Actuators B: Chemical. 212: 329-334.

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