Neuron Specific Enolase
Purified neuron specific enolase (NSE) has been prepared from human brain tissue by ion exchange chroatography and gel filtration. NSE, also known as gama enolase, 2-phospho-D-glycerate hydro-lyase, enolase-2 or Neural enolase, is a glycolytic enzyme expressed in neuronal tissue and released in response to neural tissue damage. An increase in serum levels of NSE has been found in patients with tumors such as neuroblastoma (Odelstad et al. 1981, Zeltzer et al. 1983).
- Target Species
- Product Form
- Purified enzyme from human brain - liquid
- Ion exchange chromatography and gel filtration.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- See Buffer solution.
- 50 µmol/min mg at 25ºC determined in 45 mM Imidasole, pH 6.8, 45 mM KCl, 1.33 mM Mg2SO4, 1 mM 2-PG.
- SDS PAGE: >95%
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 12 months from date of despatch.
- P09104 Related reagents
- Entrez Gene
- ENO2 Related reagents
- GO Terms
- GO:0000015 phosphopyruvate hydratase complex
- GO:0005886 plasma membrane
- GO:0000287 magnesium ion binding
- GO:0004634 phosphopyruvate hydratase activity
- GO:0006094 gluconeogenesis
- GO:0006096 glycolysis
- For research purposes only
Applications of Neuron Specific Enolase
|Application Name||Verified||Min Dilution||Max Dilution|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Neuron Specific Enolase
Hullin, D.A. et al. (1980) Purification, radioimmunoassay, and distribution of human brain 14-3-2 protein (nervous-system specific enolase) in human tissues.
Biochim Biophys Acta. 628 (1): 98-108.
Cheng, S. et al. (2015) Label-free detection of tumor markers using field effect transistor (FET)-based biosensors for lung cancer diagnosis
Sensors and Actuators B: Chemical. 212: 329-334.