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Neuron Specific Enolase

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Native Human Neuron Specific Enolase

Product Type
Purified Protein
Product Code Applications Pack Size List Price Quantity
6720-2004
Datasheet
E
SDS
50 µg loader

Purified neuron specific enolase (NSE) has been prepared from human brain tissue by ion exchange chroatography and gel filtration. NSE, also known as gama enolase, 2-phospho-D-glycerate hydro-lyase, enolase-2 or Neural enolase, is a glycolytic enzyme expressed in neuronal tissue and released in response to neural tissue damage. An increase in serum levels of NSE has been found in patients with tumors such as neuroblastoma (Odelstad et al. 1981, Zeltzer et al. 1983).

Product Details

Target Species
Human
Product Form
Purified enzyme from human brain - liquid
Preparation
Ion exchange chromatography and gel filtration.
Buffer Solution
0.1M TRIS/HCl
0.005M Magnesium Sulfate
0.25M Potassium Chloride
Preservative Stabilisers
None present
Activity
50 µmol/min mg at 25ºC determined in 45 mM Imidasole, pH 6.8, 45 mM KCl, 1.33 mM Mg2SO4, 1 mM 2-PG.
Purity
SDS PAGE: >95%
Approx. Protein Concentrations
Total protein concentration 0.96 mg/ml

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P09104
Entrez Gene
ENO2
GO Terms
GO:0000015 phosphopyruvate hydratase complex
GO:0005886 plasma membrane
GO:0000287 magnesium ion binding
GO:0004634 phosphopyruvate hydratase activity
GO:0006094 gluconeogenesis
GO:0006096 glycolysis
Regulatory
For research purposes only

Applications of Neuron Specific Enolase

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Product Specific References

References for Neuron Specific Enolase

  1. Hullin, D.A. et al. (1980) Purification, radioimmunoassay, and distribution of human brain 14-3-2 protein (nervous-system specific enolase) in human tissues.
    Biochim Biophys Acta. 628 (1): 98-108.
  2. Cheng, S. et al. (2015) Label-free detection of tumor markers using field effect transistor (FET)-based biosensors for lung cancer diagnosis
    Sensors and Actuators B: Chemical. 212: 329-334.

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