Purified neuron specific enolase
(NSE) has been prepared from human brain tissue by ion exchange chroatography and gel filtration. NSE, also known as gama enolase, 2-phospho-D-glycerate hydro-lyase, enolase-2 or Neural enolase, is a glycolytic enzyme expressed in neuronal tissue and released in response to neural tissue damage. An increase in serum levels of NSE has been found in patients with tumors such as neuroblastoma (Odelstad et al. 1981
, Zeltzer et al. 1983
- Target Species
- Product Form
- Purified enzyme from human brain - liquid
- Ion exchange chromatography and gel filtration.
- Buffer Solution
- 0.1M TRIS/HCl
0.005M Magnesium Sulfate
0.25M Potassium Chloride
- Preservative Stabilisers
- None present
- 58 µmol/min mg at 25ºC determined in 45 mM Imidasole, pH 6.8, 45 mM KCl, 1.33 mM Mg2SO4, 1 mM 2-PG.
- SDS PAGE: >95%
- Approx. Protein Concentrations
- Total protein concentration 0.87 mg/ml
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
phosphopyruvate hydratase complex
magnesium ion binding
phosphopyruvate hydratase activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Neuron Specific Enolase
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Neuron Specific Enolase
Hullin, D.A. et al. (1980) Purification, radioimmunoassay, and distribution of human brain 14-3-2 protein (nervous-system specific enolase) in human tissues.
Biochim Biophys Acta. 628 (1): 98-108.
Cheng, S. et al. (2015) Label-free detection of tumor markers using field effect transistor (FET)-based biosensors for lung cancer diagnosis
Sensors and Actuators B: Chemical. 212: 329-334.
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