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MMP-3

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Recombinant Human MMP-3

Product Type
Recombinant Protein
Specificity
MMP-3

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Recombinant Human MMP-3 Antigen produced in E. coli represents human MMP-3 (matrix metalloproteinase 3) containing the entire catalytic N-terminal domain and C-terminal domain. MMP-3 is otherwise known as stromelysin-1, a member of a family of secreted zinc-dependent endoproteases, playing a role in the degradation of extracellular matrix components, including collagens, laminin, basement membrane glycoprotein and fibronectin.

MMP-3 is implicated in wound repair, and tumor initiation, and there is high expression of MMP-3 by monocytes, and MMP-3 also expressed by microglial cells, chondrocytes, and fibroblast-like synoviocytes in patients with rheumatoid arthritis (RA) and osteoarthritis (Green et al. 2003).

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant MMP-3 expressed in E.coli
Buffer Solution
10mM Sodium Phosphate
.1% CHAPS
0.1mM Calcium Chloride
Preservative Stabilisers
None present
Carrier Free
Yes
Activity
MMP-3 activity was measured by its ability to cleave a chromogenic peptide MMP-3 substrate at room temperature. At a MMP-3 concentration of 2.5 μg/ml, 50% cleavage was achieved at an incubation time of approximately 75 mins.
Purity
>98% by SDS PAGE and HPLC
Approx. Protein Concentrations
0.5 mg/ml after reconstitution
Protein Molecular Weight
42.8 kDa (378 amino acid residues) containing the entire catalytic N-terminal and the C-terminal domain
Endotoxin Level
< 1.0 EU/ug
Regulatory
For research purposes only
Guarantee
3 months from date of reconstitution

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Synonyms
STROMELYSIN-1
UniProt
P08254
Entrez Gene
MMP3
GO Terms
GO:0005509 calcium ion binding
GO:0004222 metalloendopeptidase activity
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0006508 proteolysis
GO:0008270 zinc ion binding
GO:0030574 collagen catabolic process
GO:0043231 intracellular membrane-bounded organelle
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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