Recombinant Human MMP-1

Product Type
Recombinant Protein

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Recombinant Human MMP-1 antigen produced in E. coli corresponds to human MMP-1 (matrix metalloproteinase 1) containing the entire catalytic N-terminal domain and the C-terminal domain. MMP-1 otherwise known as interstitial collagenase, is a member of a family of secreted zinc-dependent endoproteases, which play a role in the degradation of extracellular matrix (ECM) components, including both fibrillar and non-fibrillar collagens, laminin, basement membrane glycoprotein and fibronectin.

MMP-1 specifically cleaves types I, II, III, VII and X collagens, and also interacts with and cleaves the secreted viral Tat protein during HIV infection, resulting in a decrease in neuronal Tat's mediated neurotoxicity.

MMP-1 is overexpressed in invasive melanoma (Ye et al. 2001), colorectal (Hinoda et al. 2001) and oesophageal (Yamashita et al. 2001) cancers, and has been implicated in arthritis, atherosclerotic lesion formation, and in the repair processes of the heart after myocardial infarction (Creemers et al. 2001).

Target Species
Product Form
Purified recombinant protein - lyophilized
Reconstitute with 20ul distilled water
Slow to dissolve.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed for about 10 minutes after reconstitution for better solubility.
Purified recombinant MMP-1 expressed in E.coli
Buffer Solution
Sodium phosphate
Calcium chloride
Preservative Stabilisers
None present
Carrier Free
MMP-1 activity was measured by its ability to cleave a chromogenic peptide MMP-1 substrate at room temperature. At an MMP-1 concentration of 2.5 μg/ml, 50% cleavage was achieved at an incubation time of approximately 25 mins.
>90% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.5 mg/ml after reconstitution
Endotoxin Level
< 1.0 EU/ug
For research purposes only
3 months from date of reconstitution

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Interstitial Collagenase
Entrez Gene
GO Terms
GO:0005509 calcium ion binding
GO:0007596 blood coagulation
GO:0004222 metalloendopeptidase activity
GO:0005578 proteinaceous extracellular matrix
GO:0006508 proteolysis
GO:0008270 zinc ion binding
GO:0030574 collagen catabolic process
GO:0044419 interspecies interaction between organisms
GO:0050900 leukocyte migration
View more products with MMP-1 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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