|Recombinant Human MMP-1 antigen produced in E. coli corresponds to human MMP-1 (matrix metalloproteinase 1) containing the entire catalytic N-terminal domain and the C-terminal domain. MMP-1 otherwise known as interstitial collagenase, is a member of a family of secreted zinc-dependent endoproteases, which play a role in the degradation of extracellular matrix (ECM) components, including both fibrillar and non-fibrillar collagens, laminin, basement membrane glycoprotein and fibronectin.
MMP-1 specifically cleaves types I, II, III, VII and X collagens, and also interacts with and cleaves the secreted viral Tat protein during HIV infection, resulting in a decrease in neuronal Tat's mediated neurotoxicity.
MMP-1 is overexpressed in invasive melanoma (Ye et al. 2001), colorectal (Hinoda et al. 2001) and oesophageal (Yamashita et al. 2001) cancers, and has been implicated in arthritis, atherosclerotic lesion formation, and in the repair processes of the heart after myocardial infarction (Creemers et al. 2001).
- Target Species
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitute with 20ul distilled water
Slow to dissolve.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed for about 10 minutes after reconstitution for better solubility.
- Purified recombinant MMP-1 expressed in E.coli
- Buffer Solution
Sodium phosphate Calcium chloride
- Preservative Stabilisers
- None present
- Carrier Free
- MMP-1 activity was measured by its ability to cleave a chromogenic peptide MMP-1 substrate at room temperature. At an MMP-1 concentration of 2.5 μg/ml, 50% cleavage was achieved at an incubation time of approximately 25 mins.
- >90% by SDS PAGE and HPLC analysis
- Approx. Protein Concentrations
- 0.5 mg/ml after reconstitution
- Endotoxin Level
- < 1.0 EU/ug
- For research purposes only
- 3 months from date of reconstitution
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
- Interstitial Collagenase
- Entrez Gene
- GO Terms
- GO:0005509 calcium ion binding
- GO:0007596 blood coagulation
- GO:0004222 metalloendopeptidase activity
- GO:0005578 proteinaceous extracellular matrix
- GO:0006508 proteolysis
- GO:0008270 zinc ion binding
- GO:0030574 collagen catabolic process
- GO:0044419 interspecies interaction between organisms
- GO:0050900 leukocyte migration
- View More GO Terms
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