M-CSF

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Recombinant Human M-CSF

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PHP220 Datasheet	E FN WB SDS	10 µg	Log in		Get Quote

M-CSF (Macrophage colony-stimulating factor) is a potent lineage restricted haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which plays a key role in the regulation of cellular differentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells.

Along with sRANKL/CD254/OPGL, M-CSF is essential for osteoclastogenesis, acting through the tyrosine kinase receptor c-fms (colony stimulating factor 1 receptor), and facilitates haematopoietic recovery following bone marrow transplantation.

Product Details

Target Species: Human
Product Form: Purified recombinant protein - lyophilized
Reconstitution: Reconstitute with 0.1 ml distilled water. For extended storage the addition of 0.1% BSA is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Purified recombinant M-CSF expressed in E.coli
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Carrier Free: Yes
Activity: The ED₅₀, determined by the dose-dependent stimulation of the proliferation of M-NSF-60 cells, was ≤ 1 ng/ml corresponding to a specific activity of ≥ 1 x 10⁶ units/mg.
Purity: ≥ 98% by SDS PAGE and HPLC
Approx. Protein Concentrations: 0.1 mg/ml after reconstitution
Protein Molecular Weight: 36.8 kDa (159 amino acid homodimer)
Endotoxin Level: < 1.0 EU/ug

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 3 months from date of reconstitution

More Information

UniProt: P09603
Entrez Gene: CSF1
GO Terms: GO:0005886 plasma membrane; GO:0001954 positive regulation of cell-matrix adhesion; GO:0003006 developmental process involved in reproduction; GO:0016021 integral to membrane; GO:0005125 cytokine activity; GO:0005157 macrophage colony-stimulating factor receptor binding; GO:0005615 extracellular space; GO:0008083 growth factor activity; GO:0008283 cell proliferation; GO:0010628 positive regulation of gene expression; GO:0010744 positive regulation of macrophage derived foam cell differentiation; GO:0030225 macrophage differentiation; GO:0030316 osteoclast differentiation; GO:0030335 positive regulation of cell migration; GO:0032270 positive regulation of cellular protein metabolic process; GO:0032946 positive regulation of mononuclear cell proliferation; GO:0042117 monocyte activation; GO:0042803 protein homodimerization activity; GO:0043235 receptor complex; GO:0045651 positive regulation of macrophage differentiation; GO:0045657 positive regulation of monocyte differentiation; GO:0045860 positive regulation of protein kinase activity; GO:0048471 perinuclear region of cytoplasm
Regulatory: For research purposes only

Applications of M-CSF

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.2	0.4ng/well
Functional Assays	1.0	10.0ng/ml
Western Blotting	1.5	3.0ng/lane

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: PHP220 may be used in ELISA applications with AHP1915.
Western Blotting: PHP220 may be used under reducing or non-reducing western blotting conditions with AHP1915.

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rabbit anti Human M-CSF	AHP1915	E FN P* WB	0.1 mg	Log in		Get Quote

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