M-CSF

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Recombinant Human M-CSF

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP220 E FN WB 10 µg
M-CSF (Macrophage colony-stimulating factor) is a potent lineage restricted haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which plays a key role in the regulation of cellular differentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells.

Along with sRANKL/CD254/OPGL, M-CSF is essential for osteoclastogenesis, acting through the tyrosine kinase receptor c-fms (colony stimulating factor 1 receptor), and facilitates haematopoietic recovery following bone marrow transplantation.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water. For extended storage the addition of 0.1% BSA is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant M-CSF expressed in E.coli
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
None present
Activity
The ED50, determined by the dose-dependent stimulation of the proliferation of M-NSF-60 cells, was ≤ 1 ng/ml corresponding to a specific activity of ≥ 1 x 106 units/mg.
Purity
≥ 98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1mg/ml after reconstitution.
Molecular Weight
36.8kD (159 amino acid homodimer)
Endotoxin Level
<1EU/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P09603 Related reagents
Entrez Gene
CSF1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0001954 positive regulation of cell-matrix adhesion
GO:0003006 developmental process involved in reproduction
GO:0016021 integral to membrane
GO:0005125 cytokine activity
GO:0005157 macrophage colony-stimulating factor receptor binding
GO:0005615 extracellular space
GO:0008083 growth factor activity
GO:0008283 cell proliferation
GO:0010628 positive regulation of gene expression
GO:0010744 positive regulation of macrophage derived foam cell differentiation
GO:0030225 macrophage differentiation
GO:0030316 osteoclast differentiation
GO:0030335 positive regulation of cell migration
GO:0032270 positive regulation of cellular protein metabolic process
GO:0032946 positive regulation of mononuclear cell proliferation
GO:0042117 monocyte activation
GO:0042803 protein homodimerization activity
GO:0043235 receptor complex
GO:0045651 positive regulation of macrophage differentiation
GO:0045657 positive regulation of monocyte differentiation
GO:0045860 positive regulation of protein kinase activity
GO:0048471 perinuclear region of cytoplasm
Regulatory
For research purposes only

Applications of M-CSF

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 1.0 10.0ng/ml
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
PHP220 may be used in ELISA applications with AHP1915.
Western Blotting
PHP220 may be used under reducing or non-reducing western blotting conditions with AHP1915.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Human M-CSF AHP1915 0.1 mg E FN P* WB